Amelioration of ammonia-induced intestinal oxidative stress by dietary Clostridium butyricum in giant freshwater prawn (Macrobrachium rosenbergii)

文献类型： 外文期刊

第一作者： Sun, Cunxin

作者： Sun, Cunxin;Zhou, Qunlan;Zheng, Xiaochuan;Liu, Bo;Zhao, Yongfeng;Sun, Cunxin;Tadese, Dawit Adisu;Wangari, Muritu Rebecca;Zhou, Qunlan;Zheng, Xiaochuan;Liu, Bo;Zhao, Yongfeng;Sun, Cunxin;Tadese, Dawit Adisu;Wangari, Muritu Rebecca;Zhou, Qunlan;Liu, Bo;Zhao, Yongfeng;Tadese, Dawit Adisu;Tadese, Dawit Adisu;Dagne, Adamneh;Tamiru, Metekia;Janssens, Geert Paul Jules;Tamiru, Metekia

作者机构：

关键词： Clostridium butyricum; Ammonia challenge; Antioxidant capacity; Heat -shock protein; Macrobrachium rosenbergii

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.622； 五年影响因子：4.799 ）

ISSN： 1050-4648

年卷期： 2022 年 131 卷

页码：

收录情况： SCI

摘要： The use of Clostridium butyricum in crustacean aquaculture for anti-abiotic stress is yet unknown. Feeds were formulated containing 0, 125, 250, 500, and 1000 mg/kg Clostridium butyricum (2 x 107 CFU/g), respectively. The giant freshwater prawns (Macrobrachium rosenbergii) were fed for 8 weeks in triplicate. The results showed that C. butyricum-supplemented groups improved growth performance significantly with the optimum level at 610 mg/kg. Ammonia stress reduced hemolymph glucose, total protein, total cholesterol, and triglyceride con-centrations while dietary C. butyricum significantly increased hemolymph glucose and total protein levels after the ammonia challenge. Ammonia stress increased inducible nitric oxide synthase (iNOS) and nitric oxide (NO) levels, and the treatments supplemented with C. butyricum had considerably enhanced levels of iNOS and NO after stress. Treatment with C. butyricum increased the level of superoxide dismutase (SOD), and decreased the level of malondialdehyde (MDA) and superoxide anion, with the 125 mg/kg treated groups having the extreme value. Furthermore, C. butyricum-treated groups reduced the expression of HSPs after ammonia stress while the ammonia stress induced the expression of HSP60, HSP70, and HSP90. Dietary C. butyricum elevated the expression of peroxiredoxin-5 and toll in response to ammonia stress. The results indicate that dietary supple-mentation with 125-500 mg/kg of C. butyricum (2 x 107 CFU/g) improved biochemical and antioxidant features as well as intestinal immunity of M. rosenbergii under ammonia challenge by activating the toll signal pathway.

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