OsYGL54 is essential for chloroplast development and seedling survival in rice

文献类型： 外文期刊

第一作者： Zhai, Rongrong

作者： Zhai, Rongrong;Ye, Jing;Wu, Mingming;Zhu, Guofu;Yu, Faming;Ye, Shenghai;Zhang, Xiaoming;Ye, Shenghai

作者机构：

关键词： Rice; Pale green; SRP54 protein; Chloroplast development

期刊名称：PLANT GROWTH REGULATION （ 影响因子：4.2； 五年影响因子：4.0 ）

ISSN： 0167-6903

年卷期： 2022 年 98 卷 3 期

页码：

收录情况： SCI

摘要： Chloroplast signal recognition particle (cpSRP) is composed of cpSRP54 and cpSRP43, which form a soluble transit complex with light-harvesting chlorophyll a/b-binding protein in the chloroplast stroma. cpSRP participates in the transport of chloroplast proteins to the thylakoid membranes. Rice (Oryza sativa L.) has two copies of cpSRP54s, OscpSRP54a and OscpSRP54b. In this study, we obtained knockout mutants by editing OsYGL54 (OscpSRP54b) and overexpressed plants (OEYGL54). Compared with the wild type, OsYGL54 knockout mutants had a higher degree of yellow and a lower chlorophyll content in their leaves (P < 0.05). OsYGL54 was expressed constitutively and preferentially in the leaves, and the OsYGL54 protein was localized to the chloroplasts. Abnormal and irregular chloroplasts were observed in the leaves of the OsYGL54 knockout mutants. Furthermore, the expression levels of genes related to pigment metabolism, chloroplast development, and photosynthesis, such as HEMA1, CAO1, psbO, atpA, petA, petE, Lhca1, Lhcb2.1, and Lhcb4.1 were significantly affected by OsYGL54. High chlorophyll content was detected, and the transcriptional level of OsYGL54 was elevated in the OEYGL54. Interestingly, the expression of CHLM, PSY2, atpG and chl a/b were increased in OEYGL54, but were decreased in ygl54-1. These results suggested further studies will be needed to examine cpSRP54s and to elucidate the mechanisms of this protein in rice chloroplast development. Furthermore, this study provided a theoretical basis for breeding rice varieties with high light efficiency.

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