Zinc alleviates thermal stress-induced damage to the integrity and barrier function of cultured chicken embryonic primary jejunal epithelial cells via the MAPK and PI3K/AKT/mTOR signaling pathways

文献类型： 外文期刊

第一作者： Huang, Liang

作者： Huang, Liang;Lin, Xuanxu;Lu, Lin;Zhang, Liyang;Liao, Xiudong;Cao, Chunyu;Wu, Wei;Luo, Xugang;Liu, Hsiao-Ching;Odle, Jack;See, Miles Todd

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关键词： thermal stress; zinc; integrity; barrier function; broiler intestinal epithelial cell

期刊名称：POULTRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）

ISSN： 0032-5791

年卷期： 2024 年 103 卷 6 期

页码：

收录情况： SCI

摘要： Zinc ( Zn ) could alleviate the adverse effect of high temperature ( HT ) on intestinal integrity and barrier function of broilers, but the underlying mechanisms remain unclear. We aimed to investigate the possible protective mechanisms of Zn on primary cultured broiler jejunal epithelial cells exposed to thermal stress ( TS ). In Exp.1, jejunal epithelial cells were exposed to 40 degrees C (normal temperature, NT ) and 44 degrees C ( HT ) for 1, 2, 4, 6, or 8 h. Cells incubated for 8 h had the lowest transepithelial resistance ( TEER ) and the highest phenol red permeability under HT. In Exp.2, the cells were preincubated with different Zn sources (Zn sulfate as iZn and Zn proteinate with the moderate chelation strength as oZn) and Zn supplemental levels (50 and 100 mu mol/L) under NT for 24 h, and then continuously incubated under HT for another 8 h. TS increased phenol red permeability, lactate dehydrogenase ( LDH ) activity and p-PKC/PKC level, and decreased TEER, cell proliferation, mRNA levels of claudin-1, occludin, zona occludens-1 ( ZO-1 ), PI3K, AKT and mTOR , protein levels of claudin-1, ZO-1 and junctional adhesion molecule -A (JAM -A), and the levels of p-ERK/ERK, pPI3K/PI3K and p-AKT/AKT. Under HT, oZn was more effective than iZn in increasing TEER, occludin, ZO-1, PI3K, and AKT mRNA levels, ZO-1 protein level, and p-AKT/AKT level; supplementation with 50 mu mol Zn/L was more effective than 100 mu mol Zn/L in increasing cell proliferation, JAM -A, PI3K, AKT, and PKC mRNA levels, JAM -A protein level, and the levels of pERK/ERK and p-PI3K/PI3K; furthermore, supplementation with 50 mu mol Zn/L as oZn had the lowest LDH activity, and the highest ERK, JNK-1, and mTOR mRNA levels. Therefore, supplemental Zn, especially 50 mu mol Zn/L as oZn, could alleviate the TS -induced integrity and barrier function damage of broiler jejunal epithelial cells possibly by promoting cell proliferation and tight junction protein expression via the MAPK and PI3K/AKT/mTOR signaling pathways.

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