Establishment and identification of the head kidney cell line of yellowfin seabream ( Acanthopagrus latus) ) and its application in a virus susceptibility study

文献类型： 外文期刊

第一作者： Yang, Hui-Yuan

作者： Yang, Hui-Yuan;Zhu, Ke-Cheng;Guo, Hua-Yang;Zhang, Nan;Liu, Bao-Suo;Xian, Lin;Zhu, Teng-Fei;Zhang, Dian-Chang;Yang, Hui-Yuan;Guo, Ran;Zhu, Ke-Cheng;Guo, Hua-Yang;Zhang, Nan;Liu, Bao-Suo;Xian, Lin;Zhu, Teng-Fei;Zhang, Dian-Chang;Zhu, Ke-Cheng;Guo, Hua-Yang;Zhang, Nan;Liu, Bao-Suo;Zhang, Dian-Chang

作者机构：

关键词： Acanthopagrus latus; Yellowfin seabream; Head kidney; Cell line; Immune genes; Virus

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：2.7； 五年影响因子：2.8 ）

ISSN： 0145-305X

年卷期： 2024 年 161 卷

页码：

收录情况： SCI

摘要： The yellowfin seabream ( Acanthopagrus latus) ) is a crucial marine resource owing to its economic significance. Acanthopagrus latus aquaculture faces numerous challenges from viral diseases, but a robust in-vitro research model to understand and address these threats is lacking. Therefore, we developed a novel A. latus cell line from head kidney cells called ALHK1. 1 . This study details the development, characterisation, and viral susceptibility properties of ALHK cells. This cell line primarily comprises fibroblast-like cells and has robust proliferative capacity when cultured at 28 degrees C in Leibovitz's L-15 medium supplemented with 10-20% foetal bovine serum. It exhibited remarkable stability after more than 60 consecutive passages and validation through cryopreservation techniques. The specificity of the ALHK cell line's origin from A. latus was confirmed via polymerase chain reaction (PCR) amplification of the cytochrome B gene, and a chromosomal karyotype analysis revealed a diploid count of 48 (2n = 48). Furthermore, the lipofection-mediated transfection efficiency using the pEGFP-N3 plasmid was high, at nearly 40%, suggesting that ALHK cells could be used for studies involving exogenous gene manipulation. In addition, ALHK cells displayed heightened sensitivity to the large mouth bass virus (LMBV), substantiated through observations of cytopathic effects, quantitative real-time PCR, and viral titration assays. Finally, the response of ALHK cells to LMBV infection resulted in differentially expressed antiviral genes associated with innate immunity. In conclusion, the ALHK cell line is a dependable in-vitro platform for elucidating the mechanisms of viral diseases in yellowfin seabream. Moreover, this cell line could be valuable for immunology, vaccine development, and host-pathogen interaction studies.

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