Role of PI3K/AKT signaling pathway involved in self-renewing and maintaining biological properties of chicken primordial germ cells

文献类型： 外文期刊

第一作者： Liu, Xin

作者： Liu, Xin;Ye, Liu;Ding, Ying;Gong, Wei;Qian, Hongwu;Jin, Kai;Niu, Yingjie;Zuo, Qisheng;Song, Jiuzhou;Chen, Guohong;Li, Bichun;Liu, Xin;Ye, Liu;Ding, Ying;Gong, Wei;Qian, Hongwu;Jin, Kai;Niu, Yingjie;Zuo, Qisheng;Chen, Guohong;Li, Bichun;Liu, Xin;Ye, Liu;Ding, Ying;Gong, Wei;Qian, Hongwu;Jin, Kai;Niu, Yingjie;Zuo, Qisheng;Song, Jiuzhou;Chen, Guohong;Li, Bichun;Song, Jiuzhou;Han, Wei;Li, Bichun

作者机构：

关键词： chicken primordial germ cell; PI3K/AKT; proliferation; biological property

期刊名称：POULTRY SCIENCE （ 影响因子：3.8； 五年影响因子：4.1 ）

ISSN： 0032-5791

年卷期： 2024 年 103 卷 11 期

页码：

收录情况： SCI

摘要： Avian primordial germ cells (PGCs) are important culture cells for the production of transgenic chickens and preservation of the genetic resources of endangered species; however, culturing these cells in vitro proves challenging. Although the proliferation of chicken PGCs is dependent on insulin, the underlying molecular mechanisms remain unclear. In the present study, we explored the expression of the PI3K/AKT signaling pathway in PGCs, investigated its effects on PGC self-renewal and biological properties, and identified the underlying mechanisms. Our findings indicated that although supplementation with the PI3K/AKT activator IGF-1 failed to promote proliferation under the assessed culture conditions, the PI3K/AKT inhibi tor LY294002 resulted in retarded cell proliferation and reduced expression of germ cell-related markers. We further demonstrated that inhibition of PI3K/AKT regulates the cell cycle and promotes apoptosis in PGCs by activating the expression of BAX and inhibiting that of Bcl-2. . These findings indicated that the PI3K/AKT pathway is required for cell renewal, apoptosis, and maintenance of the reproductive potential in chicken PGCs. This study aimed to provide a theoretical basis for the optimization and improvement of a culture system for chicken PGCs and provide insights into the self- renewal of vertebrate PGCs as well as potential evolutionary changes in this unique cell population.

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