Innovations in Transgene Integration Analysis: A Comprehensive Review of Enrichment and Sequencing Strategies in Biotechnology

文献类型： 外文期刊

第一作者： Zhu, Zaobing

作者： Zhu, Zaobing;Lu, Shengtao;Wang, Fan;Xu, Wenting;Yang, Litao;Zhu, Zaobing;Wang, Hongchun;Zhu, Yulei;Xue, Jing;Zhu, Zaobing;Lu, Shengtao;Yang, Litao

作者机构：

关键词： transgene DNA integration; targeted DNA enrichment; sequencing; CRISPR/Cas; pAgo

期刊名称：ACS APPLIED MATERIALS & INTERFACES （ 影响因子：8.2； 五年影响因子：8.5 ）

ISSN： 1944-8244

年卷期： 2025 年 17 卷 2 期

页码：

收录情况： SCI

摘要： Understanding the integration of transgene DNA (T-DNA) in transgenic crops, animals, and clinical applications is paramount for ensuring the stability and expression of inserted genes, which directly influence desired traits and therapeutic outcomes. Analyzing T-DNA integration patterns is essential for identifying potential unintended effects and evaluating the safety and environmental implications of genetically modified organisms (GMOs). This knowledge is crucial for regulatory compliance and fostering public trust in biotechnology by demonstrating transparency in genetic modifications. This review highlights recent advancements in T-DNA integration analysis, specifically focusing on targeted DNA enrichment and sequencing strategies. We examine key technologies, such as polymerase chain reaction (PCR)-based methods, hybridization capture, RNA/DNA-guided endonuclease-mediated enrichment, and high-throughput resequencing, emphasizing their contributions to enhancing precision and efficiency in transgene integration analysis. We discuss the principles, applications, and recent developments in these techniques, underscoring their critical role in advancing biotechnological products. Additionally, we address the existing challenges and future directions in the field, offering a comprehensive overview of how innovative DNA-targeted enrichment and sequencing strategies are reshaping biotechnology and genomics.

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