A dual-probe one-pot RPA-CRISPR/Cas12a: a highly sensitive and rapid method for detection of Phytophthora vignae

文献类型： 外文期刊

第一作者： Lin, Mingze

作者： Lin, Mingze;Zhou, Zhiming;Feng, Wanzhen;Yang, Chengdong;Chen, Qinghe;Lin, Mingze;Zhou, Zhiming;Li, Zhiting;Feng, Wanzhen;Yang, Chengdong;Chen, Qinghe;Zhu, Zaobing

作者机构：

关键词： cowpea; dual-probes; one-pot RPA-CRISPR/Cas12a; visual detection

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：3.8； 五年影响因子：4.3 ）

ISSN： 1526-498X

年卷期： 2025 年 81 卷 9 期

页码：

收录情况： SCI

摘要： BACKGROUNDPhytophthora vignae is the primary pathogen affecting cowpea plants. Early on-site and accurate detection of the causal pathogen is critical for successful management. Therefore, it is both essential and urgent to create a rapid, sensitive, and accurate detection for P. vignae in cowpeas.RESULTSIn this study, the one-pot RPA-CRISPR/Cas12a assay demonstrated the ability to effectively differentiate closely related oomycete species, exhibiting excellent specificity. The entire detection process can be completed within 15-20 min, with results being visually determined using a lateral flow strip (LFS) or by fluorescence detection under blue or ultraviolet (UV) light. Additionally, we have added dual probes to the one-pot RPA-CRISPR/Cas12a assay detection system, this modification improved reaction efficiency and significantly enhanced sensitivity (200 fg mu L-1).CONCLUSIONThis study developed a dual-probe one-pot RPA-CRISPR/Cas12a detection platform that demonstrates rapid and highly sensitive identification of P. vignae. The proposed technology enables field-deployable detection without requiring specialized technical expertise, representing a promising approach with substantial potential for practical applications. (c) 2025 Society of Chemical Industry.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序