Development of a new effective African swine fever virus vaccine candidate by deletion of the H240R and MGF505-7R genes results in protective immunity against the Eurasia strain

文献类型： 外文期刊

第一作者： Li, Jiangnan

作者： Li, Jiangnan;Song, Jie;Zhou, Shijun;Li, Shuai;Liu, Jia;Li, Tingting;Zhang, Zhaoxia;Zhang, Xianfeng;He, Xijun;Chen, Weiye;Zheng, Jun;Zhao, Dongming;Bu, Zhigao;Huang, Li;Weng, Changjiang;Li, Jiangnan;Song, Jie;Zhou, Shijun;Li, Shuai;Liu, Jia;Li, Tingting;Zhang, Zhaoxia;Zheng, Jun;Huang, Li;Weng, Changjiang

作者机构：

关键词： ASFV; H240R; MGF505-7R; attenuation; protective efficacy

期刊名称：JOURNAL OF VIROLOGY （ 影响因子：5.4； 五年影响因子：4.9 ）

ISSN： 0022-538X

年卷期： 2023 年

页码：

收录情况： SCI

摘要： African swine fever is a contagious and lethal disease of domestic pigs and wild boars, which has caused significant economic loss for the swine industry. African swine fever virus (ASFV) is the etiological agent of ASF. Until now, no effective commercial vaccine and antiviral drugs are available for ASF control. Here, we report a new designed live-attenuated mutant (ASFV-Delta H240R-Delta 7R) by deleting H240R and MGF505-7R genes based on the highly pathogenic ASFV HLJ/18 backbone. The viral titer of ASFV-Delta H240R-Delta 7R was decreased approximately 1.0 log in porcine alveolar macrophages (PAMs) compared with its parental ASFV HLJ/18, and it is highly stability following 30 serial passages in vitro. Piglets immunized by intramuscular inoculation with 10(3) or 10(5) HAD(50) of ASFV-Delta H240R-Delta 7R displayed safety without any ASF-related signs and could not be transmitted by direct contact. In addition, the immunized pigs produced specific antibodies against p30 but not those cohabitation piglets. Challenged with a virulent ASFV isolate HLJ/18, the piglets in the immunized group with 10(3) HAD(50) of ASFV-Delta H240R-Delta 7R obtained clinically significant 100% protection with mild clinical symptoms, whereas the piglets in the immunized group with 10(5) HAD(50) of ASFV-Delta H240R-Delta 7R obtained 100% protection without clinical symptoms. Moreover, the piglets in the immunized group with 10(5) HAD(50) of ASFV-Delta H240R-Delta 7R exhibited low levels of virus replication and with no observed pathological changes by postmortem and histological analysis. Overall, our results provided a new designed live vaccine candidate and rationalized the understanding of ASFV gene function, virus attenuation, and protection against ASFV infection.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序