Preparation of Monoclonal Antibody for Melamine and Development of an Indirect Competitive ELISA for Melamine Detection in Raw Milk, Milk Powder, and Animal Feeds

文献类型： 外文期刊

第一作者： Zhang, Taichang

作者： Zhang, Taichang;Meng, Meng;Xi, Rimo;Yin, Weiwei;Liu, Jinting;Li, Weihua;Liu, Wei;He, Fangyang;Wan, Yuping;Feng, Caiwei;Wang, Shanliang;Lu, Xiao

作者机构：

关键词： Melamine;drug residue;monoclonal antibody;ELISA

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

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收录情况： SCI

摘要： Melamine (MEL) has been involved in several food recalls after the discovery of severe kidney damages in children and pets poisoned by melamine-adulterated food. To detect MEL residue in foods and animal feeds, an indirect competitive ELISA (cELISA) method was developed in this study based on preparation of monoclonal antibodies (MAbs) to MEL. The immunogen was prepared by linking MEL hapten with carrier protein via carbodiimide method. The method is applicable in the range of 5.0-135.0 μg L~(-1) MEL in buffer solution, with an IC_(50) value of 22.6 ± 1.9 μg L~(-1). The MAbs showed high specificity with low cross-reactivity (<1%) toward cyanurate, ammelide, and ammeline. The method was utilized in the detection of MEL in raw milk, milk powder, and animal feeds, with detection limits of 0.1 mg L~(-1)for milk, 0.2 mg kg~(-1) for milk powder, and 0.5 mg L~(-1) for feeds. The recovery ratio was 79-110% for all matrices. The intra-assay and interassay coefficients of variation were <12.0 and <13.0%, respectively. Finally, the application of the cELISA in quantity evaluation of MEL in various feeds from local markets was evaluated and discussed.

分类号： R15

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