Analysis of lysine acetylation in tomato spot wilt virus infection in Nicotiana benthamiana

文献类型： 外文期刊

第一作者： Gong, Yanwei

作者： Gong, Yanwei;Wu, Yuanhua;Li, Ying;Wang, Fenglong;Yang, Jinguang;Liu, Dongyang;Jiang, Lianqiang;Liang, Hui

作者机构： Shenyang Agr Univ, Coll Plant Protect, Shenyang, Peoples R China;Chinese Acad Agr Sci, Tobacco Res Inst, Key Lab Tobacco Pest Monitoring Controlling & Inte, Qingdao, Peoples R China;Liangshan State Co Sichuan Prov Tobacco Co, Mile, Peoples R China

关键词： lysine acetylation; TSWV; protein modifications; Nicotiana benthamiana; RBCL; post-translational

期刊名称：FRONTIERS IN MICROBIOLOGY （ 2022影响因子：5.2； 五年影响因子：6.2 ）

ISSN：

年卷期： 2023 年 14 卷

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收录情况： SCI

摘要： IntroductionKac is a model for all acylation modification studies. Kac plays a critical role in eukaryotes and prokaryotes. It is mainly involved in six major biological functions: gene expression, signal transduction, cell development, protein conversion, metabolism, and metabolite transport. MethodWe investigated and compared the acetylation modification of proteins in healthy and tomato spot wilt virus (TSWV)-infected Nicotiana benthamiana leaves. ResultWe identified 3,418 acetylated lysine sites on 1962 proteins acetylation of proteins in the TSWV-infected and control groups were compared; it was observed that 408 sites on 294 proteins were upregulated and 284 sites on 219 proteins (involved in pentose phosphate, photosynthesis, and carbon fixation in photosynthesis) were downregulated after the infection. Overall, 35 conserved motifs were identified, of which xxxkxxxxx_K_ Rxxxxxxxxx represented 1,334 (31.63%) enrichment motifs and was the most common combination. Bioinformatic analysis revealed that most of the proteins with Kac sites were located in the chloroplast and cytoplasm. They were involved in biological processes, such as cellular and metabolic processes. DiscussionIn conclusion, our results revealed that Kac may participate in the regulation of TSWV infection in N. benthamiana.

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