Prediction of conserved and variable heat and cold stress response in maize using cis-regulatory information

文献类型： 外文期刊

第一作者： Zhou, Peng

作者： Zhou, Peng;Enders, Tara A.;Myers, Zachary A.;Magnusson, Erika;Crisp, Peter A.;Noshay, Jaclyn M.;Liang, Zhikai;Greenham, Kathleen;Springer, Nathan M.;Crisp, Peter A.;Gomez-Cano, Fabio;Grotewold, Erich;Zhou, Peng;Enders, Tara A.

作者机构：

期刊名称：PLANT CELL （ 影响因子：12.085； 五年影响因子：12.796 ）

ISSN： 1040-4651

年卷期： 2022 年 34 卷 1 期

页码：

收录情况： SCI

摘要： Changes in gene expression are important for responses to abiotic stress. Transcriptome profiling of heat- or cold-stressed maize genotypes identifies many changes in transcript abundance. We used comparisons of expression responses in multiple genotypes to identify alleles with variable responses to heat or cold stress and to distinguish examples of cis- or trans-regulatory variation for stress-responsive expression changes. We used motifs enriched near the transcription start sites (TSSs) for thermal stress-responsive genes to develop predictive models of gene expression responses. Prediction accuracies can be improved by focusing only on motifs within unmethylated regions near the TSS and vary for genes with different dynamic responses to stress. Models trained on expression responses in a single genotype and promoter sequences provided lower performance when applied to other genotypes but this could be improved by using models trained on data from all three genotypes tested. The analysis of genes with cis-regulatory variation provides evidence for structural variants that result in presence/absence of transcription factor binding sites in creating variable responses. This study provides insights into cis-regulatory motifs for heat- and cold-responsive gene expression and defines a framework for developing models to predict expression responses across multiple genotypes.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序