Fusion Partner Facilitates Expression of Cell-Penetrating Peptide L2 in Pichia pastoris

文献类型： 外文期刊

第一作者： Li, Xuan

作者： Li, Xuan;Yang, Na;Mao, Ruoyu;Hao, Ya;Teng, Da;Wang, Jianhua;Li, Xuan;Yang, Na;Mao, Ruoyu;Hao, Ya;Teng, Da;Wang, Jianhua;Li, Xuan;Yang, Na;Mao, Ruoyu;Hao, Ya;Teng, Da;Wang, Jianhua;Fang, Yuxin;Dong, Na;Shan, Anshan

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关键词： fusion expression; small ubiquitin-related modifier (SUMO); small fusion tags; cell-penetrating peptide L2; Pichia pastoris

期刊名称：ANTIBIOTICS-BASEL （ 影响因子：4.6； 五年影响因子：4.9 ）

ISSN： 2079-6382

年卷期： 2024 年 13 卷 12 期

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收录情况： SCI

摘要： Background: L2 is formed by combining the pheromone of Streptococcus agalactiae (S. agalactiae) and a cell-penetrating peptide (CPP) with cell-penetrating selectivity. L2 has more significant penetration and better specificity for killing S. agalactiae. However, the production of AMPs by chemical synthesis is always a challenge because of the production cost. Methods: This study was devoted to the heterologous expression of the cell-penetrating peptide L2 in Pichia pastoris using SUMO and a short acidic fusion tag as fusion partners, and the high-density expression of SUMO-L2 was achieved in a 5 L fermenter. Results: The results showed that SUMO-L2 expression in the 5 L fermenter reached 629 mg/L. The antibacterial activity of recombinant L2 was examined; the minimum inhibitory concentration (MICs) and minimum bactericidal concentration (MBCs) of purified L2 were 4-8 mu g/mL and 8-16 mu g/mL against S. agalactiae after 84 h of lysis with 50% formic acid. Conclusions: The findings suggest that SUMO is a suitable fusion tag to express cell-penetrating peptide L2.

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