A single-nucleotide polymorphism in PvPW1 encoding b-1,3-glucanase 9 is associated with pod width in Phaseolus vulgaris L

文献类型： 外文期刊

第一作者： Xu, Kun

作者： Xu, Kun;Zhu, Jinlong;Zhai, Hong;Yang, Qiang;Zhou, Keqin;Li, Yanhua;Xia, Zhengjun;Song, Qijian;Wu, Jing;Liu, Dajun

作者机构：

关键词： Common bean; Pod width; Fine-mapping; Marker-assisted selection; (3-1,3-glucanase 9

期刊名称：JOURNAL OF GENETICS AND GENOMICS （ 影响因子：7.1； 五年影响因子：6.4 ）

ISSN： 1673-8527

年卷期： 2024 年 51 卷 12 期

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收录情况： SCI

摘要： Pod width influences pod size, shape, yield, and consumer preference in snap beans (Phaseolus vulgaris L.). In this study, we map PvPW1, a quantitative trait locus associated with pod width in snap beans, through genotyping and phenotyping of recombinant plants. We identify Phvul.006G072800, encoding the (3-1,3-glucanase 9 protein, as the causal gene for PvPW1. The PvPW1G3555 allele is found to positively regulate pod width, as revealed by an association analysis between pod width phenotype and the PvPW1 G3555C genotype across 17 bi-parental F2 populations. In total, 97.7% of the 133 wide pod accessions carry PvPW1G3555, while 82.1% of the 78 narrow pod accessions carry PvPW1C3555, indicating strong selection pressure on PvPW1 during common bean breeding. Re-sequencing data from 59 common bean cultivars identify an 8-bp deletion in the intron linked to PvPW1C3555, leading to the development of the InDel marker of PvM436. Genotyping 317 common bean accessions with PvM436 demonstrated that accessions with PvM436247 and PvM436227 alleles have wider pods compared to those with PvM436219 allele, establishing PvM436 as a reliable marker for molecular breeding in snap beans. These findings highlight PvPW1 as a critical gene regulating pod width and underscore the utility of PvM436 in marker-assisted selection for snap bean breeding. Copyright (c) 2024, The Authors. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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