Chemosensory Protein 2 of Male Athetis lepigone Is Involved in the Perception of Sex Pheromones and Maize Volatiles

文献类型： 外文期刊

第一作者： Li, Jian-Qiao

作者： Li, Jian-Qiao;Zhu, Rui;Yao, Wei-Chen;Yu, Hui-Ping;Wang, Zhen;Sun, Xin-Yue;Yuan, Di-Hua;Sun, Yuan-Yuan;Zhu, Xiu-Yun;Zhang, Ya-Nan;Huang, Jian-Rong;Emam, Sekina S.;Dewer, Youssef

作者机构：

关键词： Athetis lepigone; chemosensory protein; sex pheromone; maize volatile; site-directed mutagenesis; RNAi

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2023 年 71 卷 16 期

页码：

收录情况： SCI

摘要： In moths, the interactions between chemosensory proteins (CSPs) and sex pheromones have yet to be comprehensively investigated. Here, we examined the function of AlepCSP2 in male Athetis lepigone based on protein expression, molecular docking, site-directed mutagenesis, fluorescence competitive binding analyses, and RNA interference (RNAi) experiments. We found that AlepCSP2 showed strong binding affinity for two sex pheromones and five maize volatiles and that binding was optimal under neutral conditions. Furthermore, we identified six amino acids as being key residues involved in the interaction between AlepCSP2 and multiple ligands. Further RNAi showed that siCSP2 males displayed consistently lower electro-antennography responses to two sex pheromones and three maize volatiles at different dosages tested, and the mating rate also decreased significantly by 37.50%. These findings will contribute to characterizing the binding mechanisms of moth CSPs to sex pheromones and host volatiles and also identify unique targets for developing novel pest behavior disruptors.

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