Ultrasound-Assisted Enzyme Extraction, Physicochemical Properties and Antioxidant Activity of Polysaccharides from Cordyceps militaris Solid Medium

文献类型： 外文期刊

第一作者： Wang, Xiaoya

作者： Wang, Xiaoya;Zhang, Jingyan;Zhang, Kang;Guo, Zhiting;Xu, Guowei;Huang, Liping;Wang, Lei;Li, Jianxi

作者机构：

关键词： Cordyceps militaris solid medium; ultrasound-assisted enzyme; polysaccharides; properties; antioxidant

期刊名称：MOLECULES （ 影响因子：4.6； 五年影响因子：5.0 ）

ISSN：

年卷期： 2024 年 29 卷 19 期

页码：

收录情况： SCI

摘要： Cordyceps militaris solid medium polysaccharides (CMMPs) were extracted using an ultrasound-assisted enzyme method, and the process conditions were optimized via response surface methodology (RSM). The CMMPs were separated into four components named CMMP-1, CMMP-2, CMMP-3 and CMMP-4 using ethanol fractional precipitation, and their monosaccharide composition and structural properties were analyzed by molecular weight analysis, Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), Congo red test, ultraviolet-visible spectroscopy (UV-vis), atomic force microscopy (AFM), and thermogravimetric analysis (TGA). RSM could predict the yield of the CMMP (R-2 = 0.9928), and the polysaccharide yield was 15.43% under the selected conditions of 3.1% cellulase enzyme addition, a liquid-solid ratio of 42:1, an extraction temperature of 61 degrees C, and an extraction time of 60 min. Glucose and galactose were the main constituents of the four fractional precipitated polysaccharides. Furthermore, four components exhibited antioxidant activity, and CMMP-1 demonstrated stronger antioxidant activity in vitro. This study demonstrates the possibility of developing a natural antioxidant food from Cordyceps militaris solid medium.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序