Kojic Acid Showed Consistent Inhibitory Activity on Tyrosinase from Mushroom and in Cultured B16F10 Cells Compared with Arbutins

文献类型： 外文期刊

第一作者： Wang, Wei

作者： Wang, Wei;Gao, Ying;Wang, Weiwei;Zhang, Jianyong;Yin, Junfeng;Le, Ting;Xue, Jinjin;Jiang, Heyuan;Wang, Wei;Le, Ting;Engelhardt, Ulrich H.

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关键词： tyrosinases; kojic acid; alpha-arbutin; beta-arbutin; deoxyarbutin; monophenolase activity; diphenolase activity; inhibition type; melanin content; cell viability

期刊名称：ANTIOXIDANTS （ 影响因子：7.675； 五年影响因子：7.886 ）

ISSN：

年卷期： 2022 年 11 卷 3 期

页码：

收录情况： SCI

摘要： Kojic acid, beta-arbutin, alpha-arbutin, and deoxyarbutin have been reported as tyrosinase inhibitors in many articles, but some contradictions exist in their differing results. In order to provide some explanations for these contradictions and to find the most suitable compound as a positive control for screening potential tyrosinase inhibitors, the activity and inhibition type of the aforementioned compounds on monophenolase and diphenolase of mushroom tyrosinase (MTYR) were studied. Their effects on B16F10 cells melanin content, tyrosinase (BTYR) activity, and cell viability were also exposed. Results indicated that oc-arbutin competitively inhibited monophenolase activity, whereas they uncompetitively activated diphenolase activity of MTYR. beta-arbutin noncompetitively and competitively inhibited monophenolase activity at high molarity (4000 mu M) and moderate molarity (250-1000 mu M) respectively, whereas it activated the diphenolase activity of MTYR. Deoxyarbutin competitively inhibited diphenolase activity, but could not inhibit monophenolase activity and only extended the lag time. Kojic acid competitively inhibited monophenolase activity and competitive-noncompetitive mixed-type inhibited diphenolase activity of MTYR. In a cellular experiment, deoxyarbutin effectively inhibited BTYR activity and reduced melanin content, but it also potently decreased cell viability. oc-arbutin and beta-arbutin dose-dependently inhibited BTYR activity, reduced melanin content, and increased cell viability. Kojic acid did not affect cell viability at 43.8-700 mu M, but inhibited BTYR activity and reduced melanin content in a dose-dependent manner. Therefore, kojic acid was considered as the most suitable positive control among these four compounds, because it could inhibit both monophenolase and diphenolase activity of MTYR and reduce intercellular melanin content by inhibiting BTYR activity without cytotoxicity. Some explanations for the contradictions in the reported articles were provided.

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