Identification of monoclonal antibody targeting epitope on p72 trimeric spike of African swine fever virus

文献类型： 外文期刊

第一作者： Duan, Xulai

作者： Duan, Xulai;Liu, Yingnan;Chen, Zongyan;Xie, Zhenhua;Tian, Chuanwen;Li, Yao;Lv, Lu;Wang, Rongrong;Liu, Jingyi;Chen, Hongjun;Liu, Yingnan;Chen, Zongyan;Liu, Jingyi;Chen, Hongjun

作者机构：

关键词： African swine fever virus (ASFV); p72 protein; Monoclonal antibody; Epitope

期刊名称：VIRUS GENES （ 影响因子：1.6； 五年影响因子：2.0 ）

ISSN： 0920-8569

年卷期： 2023 年 59 卷 4 期

页码：

收录情况： SCI

摘要： African swine fever virus (ASFV) is highly contagious and can cause lethal disease in pigs. ASFV p72 protein is a major capsid protein that presents as trimer in the virion. Epitopes on the surface of p72 trimer are considered as protective antigens. In this study, recombinant p72 protein and p72-baculovirus were constructed and obtained. Three monoclonal antibodies (mAbs) specific to ASFV p72 protein, designated as 1A3, 2B5 and 4A5, were generated. Among them, 4A5 showed strong reactivity with ASFV infected cells. Subsequently, the epitope recognized by 4A5 was mapped and identified using a series of overlapping peptides generated from p72 protein. IFA and western blot analyses showed that 4A5 recognized the linear epitope of p72 monomer located between amino acids 245-285 and recognized the conformational epitope located at the surface and top of the p72 trimer. These findings will enrich our knowledge regarding the epitope on p72 protein and provide valuable information for further characterization of the antigenicity and molecular functions of p72 protein.

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