Comprehensive Transcriptome and Metabolome Characterization of Peony 'Coral Sunset' Petals Provides Insights into the Mechanism of Pigment Degradation

文献类型： 外文期刊

第一作者： Zhang, Hechen

作者： Zhang, Hechen;Yuan, Xin;Wang, Rui;Wang, Limin;Gao, Jie;Wang, Huijuan;Li, Yanmin;Fu, Zhenzhu

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关键词： anthocyanins; carotenoids; high-throughput sequencing; Paeonia lactiflora; pigment degradation

期刊名称：HORTICULTURAE （ 影响因子：3.1； 五年影响因子：3.4 ）

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年卷期： 2023 年 9 卷 12 期

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收录情况： SCI

摘要： The petals of Paeonia lactiflora 'Coral Sunset' change color from coral pink to pale yellow after flower opening. Pigment-targeted metabolomic analysis showed that the carotenoid and anthocyanin contents rapidly decreased after petal fading. SMART-sequencing and next-generation-sequencing analyses were performed to identify differentially expressed transcripts to characterize the candidate genes involved in petal fading. The expression of certain genes associated with anthocyanin and carotenoid synthesis and degradation was correlated with the petal-fading phenotype. The anthocyanin synthesis (AS) structural genes, CHS, F3H, F3 ' H, DFR, and ANS, and the carotenoid synthesis genes, LCYB and LCYE, were strongly expressed before fading, but their expression significantly declined after fading. In contrast, the expression of certain genes associated with oxidase activity and light signaling significantly increased after fading. Therefore, inhibition of pigment synthesis and accelerated pigment degradation may be crucial for petal fading. A R2R3-MYB family member of subgroup 4 (MYBs-SG4) showed the same expression pattern as the AS structural genes and functioned in the positive regulation of anthocyanin synthesis by forming the MBW protein complex. This is the first report of a SG4 member with a positive regulatory function. This study provides a foundation for elucidation of the mechanisms of pigment synthesis and metabolism, and a theoretical basis for flower-color-directed breeding.

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