Evaluation of allergenic protein profiles in three Chinese high-oleic acid peanut cultivars using NanoLC-Orbitrap mass spectrometry
文献类型： 外文期刊
第一作者： Yang, Xinke
作者： Yang, Xinke;Bai, Haochen;Yin, Lijun;Xue, Wentong;Jia, Xin;Wang, Jin;Xue, Wentong;Jia, Xin
作者机构：
关键词： Allergy; High-oleic acid peanut; Peptide-filtering pipeline; NanoLC-Orbitrap mass spectrometry
期刊名称：FOOD SCIENCE AND HUMAN WELLNESS （ 影响因子：7.0； 五年影响因子：8.3 ）
ISSN：
年卷期： 2023 年 12 卷 3 期
页码：
收录情况： SCI
摘要： High oleic-acid peanuts are known for their pre-longed shelf-life and health benefit due to high content of oleic fatty acid. However, the allergenicity and allergenic protein profiles in Chinese high-oleic peanuts have yet to be studied. For this purpose, an Orbitrap Fusion mass spectrometry (MS)-based method that is feasible for identification of putative allergenic protein as well as semi-quantitation of five major allergen protein in three different Chinese high-oleic peanut cultivars (JH 13, JH 16 and JH 18) have been reported. Results show that three Chinese high-oleic acid peanut cultivars selected all contained highly allergenic proteins Ara h 1, Ara h 2, Ara h 3 and Ara h 6. The allergenic protein profiles of Chinese high-oleic acid peanut cultivars were very similar to that of conventional peanuts, but the allergenic protein subunits varied greatly among higholeic peanuts. Additionally, a comprehensive peptide-filtering pipeline had been developed for identification of potential peptide markers in peanut allergen proteins. Through the peptide-filtering pipeline, three novel peptide markers, IVQIEAKPNTLVLPK, SSNPDIYNPQAGSLR and AQSENYEYLAFK surrogate to Ara h 1, Ara h 3 with high abundance, good MS response and highly reliability were identified, which can be used as candidate peptide markers for the detection of peanut allergens in different food matrices. (c) 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
分类号：
- 相关文献
作者其他论文 更多>>
-
Immunoprotective effects of DNA vaccine against Eimeria tenella based on EtAMA3 and EtRON2L2
作者：Chen, Xiangjie;Qiu, Xixi;Ni, Junli;Liao, Shenquan;Qi, Nanshan;Li, Juan;Lv, Minna;Lin, Xuhui;Cai, Haiming;Hu, Junjing;Song, Yongle;Yin, Lijun;Zhu, Yibin;Zhang, Jianfei;Zhang, Haoji;Sun, Mingfei
关键词：Eimeria tenella; DNA vaccine; Immunoprotective efficacy
-
Selection of a suitable reference gene for gene-expression studies in Trichomonas gallinae under various biotic and abiotic stress conditions
作者：Cai, Haiming;Zhu, Yibin;Liu, Yu;Liao, Shenquan;Li, Juan;Lv, Minna;Lin, Xuhui;Hu, Junjing;Song, Yongle;Chen, Xiangjie;Yin, Lijun;Zhang, Jianfei;Qi, Nanshan;Sun, Mingfei;Yan, Zhuanqiang;Fang, Siyun;Wang, Dingai;Shen, Hanqin
关键词：Trichomonas gallinae; Reference genes selection; Stability analysis; TgaAtg8 gene
-
Comparison of soluble dietary fibers from various quinoa microgreens: Structural characteristics and bioactive properties
作者：Wu, Ding -Tao;Li, Jie;Wang, Jin;Lei, Jing;Hu, Yi-Chen;Wu, Xiao-Yong;Zou, Liang;Wu, Ding -Tao;Li, Jie;Wang, Jin;Lei, Jing;Gan, Ren-You;Qin, Peiyou
关键词：Quinoa microgreen; Soluble dietary fiber; Pectic -polysaccharide; Molecular weights; Constituent monosaccharide; Antioxidant effect; Antiglycation effect; Prebiotic effect; Immunoregulatory effect
-
Epidemiological investigation of coccidiosis and associated risk factors in broiler chickens immunized with live anticoccidial vaccines in China
作者：Liao, Shenquan;Lin, Xuhui;Li, Juan;Lv, Minna;Hu, Junjing;Cai, Haiming;Song, Yongle;Chen, Xiangjie;Zhu, Yibin;Yin, Lijun;Zhang, Jianfei;Qi, Nanshan;Sun, Mingfei;Zhou, Qingfeng;Wang, Zhanxin;Yan, Zhuanqiang;Wang, Dingai;Su, Guanzhi
关键词：broiler chicken; coccidiosis; Eimeria spp.; prevalence; risk factors
-
Mechanism of enhancing rehydration capacity and foaming property of spray-dried egg white powder by lactic acid fermentation
作者：Jia, Jie;Duan, Jiayi;Jia, Xin;Ge, Junlin;Dong, Mengxue;Liu, Xuebo;Duan, Xiang;Yang, Xiujuan
关键词：Spray drying; Lactic acid bacteria; Surface activity; Microstructure; Contact angle
-
Alleviating Pentatrichomonas hominis-induced damage in IPEC-J2 cells: the beneficial influence of porcine-derived lactobacilli
作者：Zhu, Yibin;Cai, Haiming;Liao, Shenquan;Qi, Nanshan;Lv, Minna;Lin, Xuhui;Hu, Junjing;Song, Yongle;Chen, Xiangjie;Yin, Lijun;Zhang, Jianfei;Li, Juan;Sun, Mingfei;Yan, Zhuanqiang;Fang, Siyun;Wang, Dingai;Shen, Hanqin
关键词：Pentatrichomonas hominis; Pigs; Probiotics; Anti-oxidative response; Inflammatory response; Apoptosis
-
Development of a TaqMan polymerase chain reaction detection method for the precise identification and quantification of an attenuated Eimeria maxima vaccine strain in poultry
作者：Jin, Haozhan;Cai, Haiming;Liao, Shenquan;Qi, Nanshan;Li, Juan;Lv, Minna;Lin, Xuhui;Hu, Junjing;Song, Yongle;Zhu, Yibin;Chen, Xiangjie;Yin, Lijun;Zhang, Xiaohui;Zhang, Jianfei;Sun, Mingfei;Jin, Haozhan;Zhang, Xu
关键词：avian coccidiosis; Eimeria maxima; attenuated live oocyst vaccines; molecular marker; TaqMan PCR
