A novel aldo-keto reductase gene, OsAKR1 , from rice confers higher tolerance to cadmium stress in rice by an in vivo reactive aldehyde detoxification

文献类型： 外文期刊

第一作者： Guo, Rui

作者： Guo, Rui;Chen, Chang Zhao;Tu, Chun Yan;Shen, Ren Fang;Zhu, Xiao Fang;Guo, Rui;Zhang, Qiang;Chen, Chang Zhao;He, Meng Xing;Zhang, Qiang;Sun, Jie Ya;Huang, Jiu

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关键词： Cd stress; Genes; Methylglyoxal (MG); OsAKR1; Reactive oxygen species (ROS); Rice

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：13.6； 五年影响因子：12.7 ）

ISSN： 0304-3894

年卷期： 2024 年 470 卷

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收录情况： SCI

摘要： Elevated levels of cadmium (Cd) have the ability to impede plant development. Aldo-keto reductases (AKRs) have been demonstrated in a number of plant species to improve tolerance to a variety of abiotic stresses by scavenging cytotoxic aldehydes; however, only a few AKRs have been identified to improve Cd tolerance. The OsAKR1 gene was extracted and identified from rice here. After being exposed to Cd, the expression of OsAKR1 dramatically rose in both roots and shoots, although more pronounced in roots. According to a subcellular localization experiment, the nucleus and cytoplasm are where OsAKR1 is primarily found. Mutants lacking OsAKR1 exhibited Cd sensitive phenotype than that of the wild -type (WT) Nipponbare (Nip), and osakr1 mutants exhibited reduced capacity to scavenge methylglyoxal (MG). Furthermore, osakr1 mutants exhibited considerably greater hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA) levels, and increased catalase (CAT) activity in comparison to Nip. The expression of three isomeric forms of CAT was found to be considerably elevated in osakr1 mutants during Cd stress, as demonstrated by quantitative real-time PCR analysis, when compared to Nip. These results imply that OsAKR1 controlled rice ' s ability to withstand Cd by scavenging harmful aldehydes and turning on the reactive oxygen species (ROS) scavenging mechanism.

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