Ectopic expression of peanut acyl carrier protein in tobacco alters fatty acid composition in the leaf and resistance to cold stress

文献类型： 外文期刊

第一作者： Tang, G. -Y.

作者： Tang, G. -Y.;Liu, Z. -J.;Bi, Y. -P.;Shan, L.;Tang, G. -Y.;Liu, Z. -J.;Bi, Y. -P.;Shan, L.;Tang, G. -Y.;Liu, Z. -J.;Bi, Y. -P.;Shan, L.;Wei, L. -Q.

作者机构：

关键词： antisense; Arabidopsis thaliana; Nicotiana tabacum; overexpression; transgenic tobacco

期刊名称：BIOLOGIA PLANTARUM （ 影响因子：1.747； 五年影响因子：2.146 ）

ISSN： 0006-3134

年卷期： 2012 年 56 卷 3 期

页码：

收录情况： SCI

摘要： Acyl carrier protein (ACP), as an essential protein cofactor, plays an important role in de novo synthesis of fatty acids in plastids. In this study, the expression profile of peanut (Arachis hypogaea) AhACP1-1 and AhACP1-2 was analyzed in different tissues. The expression level of AhACP1-1 was highest in the seed, whereas expression was barely detected in the shoot, and AhACP1-2 was expressed in every tissue analyzed with the highest expression level detected in the leaf and seed. Overexpression (OE) and antisense-inhibition (AT) of AhACP1 in transgenic tobacco modified the transcript level of endogenous NtACPs, and the content of total lipids and composition of fatty acid in leaves were altered compared with the wild-type control. Transgenic OE-AhACP1 or AT-AhACP1 tobacco exhibited a significant increase or decrease in polyunsaturated C18:2 and C18:3 fatty acid content, and were more tolerant or sensitive to cold stress, respectively. It is suggested that AhACP1 bound with C18:1 might be the specific substrate of oleoyl-ACP thioesterase or glycerol-3-phosphate acyltransferase, and participates in membrane lipid synthesis.

分类号： Q94

  • 相关文献
作者其他论文 更多>>

微信小程序