Heterologous expression of two Glycine max omega-3 fatty acid desaturases in Saccharomyces cerevisiae

文献类型: 外文期刊

第一作者: Zhang, H. T.

作者: Zhang, H. T.;Bi, Y. P.;Liu, Z. J.;Shan, L.

作者机构:

关键词: Glycine max;Saccharomyces cerevisiae;omega-3 fatty acid desaturase;alpha-linolenic acid

期刊名称:RUSSIAN JOURNAL OF PLANT PHYSIOLOGY ( 影响因子:1.481; 五年影响因子:1.608 )

ISSN: 1021-4437

年卷期: 2009 年 56 卷 4 期

页码:

收录情况: SCI

摘要: alpha-Linolenic acid (ALA, C 18:3(Delta 9,12,15)) has many important biological functions. omega-3 Fatty acid desaturase (FAD3), existing in cytosolic and plastidic compartments of higher plants, catalyzes linoleic acid (LA) desaturation to produce ALA. GmFAD3A-2 and GmFAD3C genes encoding cytosolic FAD3 from Qihuang 29 soybean were cloned and inserted into p416 vector and expressed in K601 yeast strain. Gas chromatography showed that the transformed yeast strains could produce ALA. The ALA accumulation levels for the strains transformed with GmFAD3A-2 or GmFAD3C genes were 0.77 +/- 0.1 and 4.13 +/- 0.4% of total fatty acids, respectively, while, as compared with that of the control, the contents of LA decreased from 14.34 +/- 0.8 to 10.93 +/- 0.0 and 7.85 +/- 0.1%, respectively, implying that the GmFAD3C enzyme is more vigorous or stable, than GmFAD3A-2.

分类号:

  • 相关文献

[1]Transcriptomic and Physiological Evidence for the Relationship between Unsaturated Fatty Acid and Salt Stress in Peanut. Sui, Na,Wang, Yu,Liu, Shanshan,Yang, Zhen,Wang, Fang,Wan, Shubo. 2018

[2]Chronic alpha-linolenic acid treatment alleviates age-associated neuropathology: Roles of PERK/eIF2 alpha signaling pathway. Gao, Hui,Yan, Peipei,Han, Hao,Yang, Wei,Yao, Ping,Liu, Liegang,Gao, Hui,Yan, Peipei,Zhang, Shun,Han, Hao,Yang, Wei,Yao, Ping,Liu, Liegang,Nie, Shuke,Huang, Fenghong,Deng, Qianchun,Huang, Qingde,Xu, Jiqu,Huang, Fenghong,Deng, Qianchun,Huang, Qingde,Xu, Jiqu,Wu, Hailei,Ye, Keqiang. 2016

[3]Clone and identification of bifunctional Delta 12/Delta 15 fatty acid desaturase LKFAD15 from Lipomyces kononenkoae. Zhang Yan,Liang Zhuo,Jiang Mulan,Wan Xia,Gon Yangmin,Zhang Yinbo,Huang Fenghong,Zhang Yan,Huang Fenghong. 2013

[4]Effectiveness of rubber seed oil and flaxseed oil to enhance the alpha-linolenic acid content in milk from dairy cows. Pi, Y.,Gao, S. T.,Ma, L.,Wang, J. Q.,Zhang, J. M.,Xu, J. C.,Bu, D. P.,Ma, L.,Bu, D. P.,Ma, L.,Bu, D. P.,Zhu, Y. X.,Xu, J. C.,Bu, D. P.,Xu, J. C..

[5]Duodenal infusion of alpha-linolenic acid affects fatty acid metabolism in the mammary gland of lactating dairy cows. Yang, G.,Bu, D. P.,Wang, J. Q.,Khas-Erdene,Zhou, L. Y.,Loor, J. J.,Loor, J. J..

[6]Converting defatted silkworm pupae by Yarrowia lipolytica for enhanced lipid production. Shi, Xin-Yi,Li, Tai-Ying,Wang, Min,Wu, Wei-Wei,Li, Wen-Jing,Wu, Qiong-Ying,Wu, Fu-An,Wang, Jun,Wu, Qiong-Ying,Wu, Fu-An,Wang, Jun.

[7]Effects of infusing different doses of free alpha-linolenic acid to the duodenum on the immune function of lactating dairy cows.. Sun, P.,Wang, J. Q.,Yang, G.,Khas-Erdene. 2010

[8]Simultaneous improvement in production of microalgal biodiesel and high-value alpha-linolenic acid by a single regulator acetylcholine. Parsaeimehr, Ali,Sun, Zhilan,Chen, Yi-Feng,Dou, Xiao. 2015

[9]Short communication: Responses to increasing amounts of free alpha-linolenic acid infused into the duodenum of lactating dairy cows. Khas-Erdene,Wang, J. Q.,Bu, D. P.,Wang, L.,Liu, Q. S.,Yang, G.,Wei, H. Y.,Zhou, L. Y.,Drackley, J. K.. 2010

[10]SHORT COMMUNICATION: Effects of duodenal infusion of increasing amounts of alpha-linolenic acid on composition and susceptibility to peroxidation of blood lipids in lactating dairy cows. Sun, Peng,Wang, Jia-Qi,Liu, Qing-Sheng,Khas-Erdene,Yang, Guang. 2012

[11]Enrichment process for alpha-linolenic acid from silkworm pupae oil. Wang, Jun,Zhang, Jin-Liang,Wu, Fu-An,Wu, Fu-An. 2013

[12]Effect of Increasing Doses of Linoleic and alpha-Linolenic Acids on High-Fructose and High-Fat Diet Induced Metabolic Syndrome in Rats. Zhang, Jianan,Wang, Ou,Guo, Yingjian,Wang, Siyi,Li, Guopeng,Ji, Baoping,Wang, Tuo,Ji, Baoping,Deng, Qianchun,Deng, Qianchun.

[13]Duodenal infusion of alpha-linolenic acid affect fatty acids metabolism in mammary gland of lactating dairy cows. Yang, G.,Wang, J. Q.,Bu, D. P.,Khas-Erdene,Liu, Q. S.,Zhou, L. Y.,Sun, P.,Liu, K. L.. 2010

[14]A new isolation method of beta-D-glucans from spent yeast Saccharomyces cerevisiae. Liu, Xiao-Yorig,Wang, Qlang,Cui, Steve W.,Liu, Hong-Zhi. 2008

[15]Effect of yeast Saccharomyces cerevisiae supplementation on serum antioxidant capacity, mucosal sIgA secretions and gut microbial populations in weaned piglets. Zhu Cui,Wang Li,Wei Shao-yong,Ma Xian-yong,Zheng Chun-tian,Jiang Zong-yong,Zhu Cui,Chen Zhuang,Jiang Zong-yong. 2017

[16]Effects of different forms of yeast Saccharomyces cerevisiae on growth performance, intestinal development, and systemic immunity in early-weaned piglets. Jiang, Zongyong,Wei, Shaoyong,Wang, Zhilin,Hu, Shenglan,Zheng, Chuntian,Hu, Youjun,Wang, Li,Ma, Xianyong,Yang, Xuefen,Jiang, Zongyong,Zhu, Cui,Chen, Zhuang. 2015

[17]Efficient removal of zinc by multi-stress-tolerant yeast Pichia kudriavzevii A16. Li, Chunsheng,Yu, Jinzhi,Wang, Dongfeng,Xu, Ying,Li, Chunsheng,Li, Laihao,Yang, Xianqing,Ma, Haixia.

[18]Five Fatty Acyl-Coenzyme A Reductases Are Involved in the Biosynthesis of Primary Alcohols in Aegilops tauschii Leaves. Wang, Meiling,Wu, Hongqi,Li, Chunlian,Wang, Yong,Wang, Zhonghua,Xu, Jing. 2017

[19]Biosynthesis of 2-phenylethanol using tobacco waste as feedstock. Wang, Qian,Song, Yufeng,Jin, Yirong,Liu, Haobao,Sun, Yuhe,Liu, Guanshan,Zhang, Haibo.

[20]Statistical Optimization of Culture Media and Conditions for Production of Mannan by Saccharomyces cerevisiae. Liu, Hong-Zhi,Wang, Qiang,Liu, Yuan-Yuan,Fang, Fang.

作者其他论文 更多>>