文献类型： 外文期刊
第一作者： Zheng, L.
作者： Zheng, L.;Wang, Y.;Wan, S. B.;Bi, Y. P.;Zheng, L.;Peng, Z. Y.;Wang, Y.;Bian, F.;Wan, S. B.;Bi, Y. P.;Zheng, L.;Peng, Z. Y.;Wang, Y.;Bian, F.;Wan, S. B.;Bi, Y. P.;Jiao, Q. Q.;Qu, S. J.;Wan, S. B.;Bi, Y. P.
作者机构：
关键词： Nicotiana tabacum; diabetes; PROINSULIN; CRE/loxP; marker-free; transgenic tobacco plant
期刊名称：RUSSIAN JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：1.481； 五年影响因子：1.608 ）
ISSN： 1021-4437
年卷期： 2016 年 63 卷 5 期
页码：
收录情况： SCI
摘要： The demand for INSULIN is increasing rapidly along with the increased number of diabetic patients. Using the CRE/loxP system, we developed a selective marker-free system without crossing to produce PROINSULIN in transgenic plant. In frame of this approach, the induced promoter pRD29A was isolated from Arabidopsis. The CRE recombinase gene was placed under the control of pRD29A between two loxP recombination sites together with the selective NPTII gene. Furthermore, the binary vector with CRE recombinase and PROINSULIN was constructed and introduced into tobacco (Nicotiana tabacum L.) by Agrobacterium-mediated transformation. Gene excision was used to remove the sequence between the two loxP sites at the presence of 200 mM NaCl. PCR analysis showed that self-excision occurred in several T-0 transgenic plants. Transgenic plants without any marker gene successfully expressed PROINSULIN. This auto-excision strategy provides efficient means of removing the selectable marker gene from transgenic plants. It is an efficient method for producing bio-safe recombinant protein and other valuable substances in plants.
分类号： Q94
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