High efficiency in vitro plant regeneration from epicotyl explants of Chinese peanut cultivars

文献类型： 外文期刊

第一作者： Shan, Lei

作者： Shan, Lei;Tang, Guiying;Xu, Pingli;Liu, Zhanji;Bi, Yuping;Shan, Lei;Tang, Guiying;Xu, Pingli;Liu, Zhanji;Bi, Yuping;Shan, Lei;Tang, Guiying;Xu, Pingli;Liu, Zhanji;Bi, Yuping

作者机构：

关键词： Peanut (Arachis hypogaea L.); Regeneration; Multiple shoot formation; Epicotyl explant

期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT （ 影响因子：2.252； 五年影响因子：2.139 ）

ISSN： 1054-5476

年卷期： 2009 年 45 卷 5 期

页码：

收录情况： SCI

摘要： An efficient organogenesis and micropropagation system was developed for in vitro plant regeneration of multiple cultivars of peanut (Arachis hypogaea). The system was used to regenerate plants from nine cultivars: Luhua no. 9, Luhua no. 13, Luhua no. 14, Fenghua no. 1, Fenghua no. 3, Fenghua no. 5, Huayu no. 23, Qinglan no. 2, and Baisha 1016. Epicotyl and embryo axis explants were cultured on Murashige and Skoog (MS) basal medium supplemented with 33.29-44.39 mu M 6-benzyladenine (BAP) and 2.15-4.30 mu M alpha-naphthaleneacetic acid (NAA). The highest rate of shoot formation was observed in epicotyl explants taken from 4-d-old seedlings (5.1 +/- 1.4 shoots per explant). Optimum shoot development was observed in explants cultured on MS medium containing 4.48 mu M BAP and 2.89 or 5.78 mu M gibberellin (GA(3)). Well-developed shoots (3-5 cm high) formed roots after 2 wk on MS medium containing 0-2.69 mu M NAA. We observed that all multiple shoots formed at the site of epicotyl incision and at the upper end of each section, indicating physiological polarity of shoot formation. The maximum shoot induction rate for Luhua no. 9, Luhua no. 13, Luhua no. 14, Fenghua no. 1, Fenghua no. 3, Fenghua no. 5, Huayu no. 23, Qinglan no. 2, and Baisha 1016 was 60.0%, 83.3%, 80.7%, 91.5%, 86.0%, 59.7%, 75.0%, 67.3%, and 72.7%, respectively. This regeneration system will play a vital role in achieving the genetic improvement of Chinese peanut.

分类号： Q94

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