A delta-endotoxin encoded in Pseudomonas fluorescens displays a high degree of insecticidal activity

文献类型: 外文期刊

第一作者: Peng, R

作者: Peng, R;Xiong, A;Li, X;Fuan, H;Yao, Q

作者机构:

关键词: Bacteriophage-t7 rna-polymerase;Bacillus-thuringiensis toxins;Gram-negative bacteria;Escherichia-coli;Crystal proteins;Fusarium-wilt;Foreign genes;Expression;Resistance;Promoter

期刊名称:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY ( 影响因子:4.813; 五年影响因子:4.697 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: The short field-life of Bacillus thuringiensis (Bt) insecticidal crystal protein has limited its use. When the Bt toxin is produced in Pseudomonas fluorescens it can be encapsulated and retain its effectiveness for two to three times longer than other Bt formulations. In order to improve Bt expression, we have synthesized cryIA(c) Bt delta-endotoxin encoding region (GenBank AF537267) according to the usage codon of P. fluorescens and transformed the Bt toxin expression cassette into P. fluorescens strains. T7 RNA polymerase and the T7 promoter system were used to control expression of Bt toxin. SDS-PAGE and Western blotting assay revealed that the delta-endotoxin was expressed as 8% of the total protein in P. fluorescens. In in vitro tests, release of toxin from dead bacteria was demonstrated. Supplementation of diets with Bt toxin-containing Pseudomonas bacterium resulted in high mortality of cabbage butterfly (Pieris brassicae) larvae. [References: 44]

分类号: Q93

  • 相关文献

[1]A Na+/H+ antiporter gene of the moderately halophilic bacterium Halobacillus dabanensis D-8(T): cloning and molecular characterization. Yang, LF,Jiang, JQ,Zhao, BS,Zhang, B,Feng, DQ,Lu, WD,Wang, L,Yang, SS.

[2]Renaturation and purification of ApxII toxin of Actinobacillus pleuropneumoniae. Wang, Chunlai,Liu, Siguo,Peng, Yonggang,Shao, Meili,Wang, Yong,Gong, Qiang,Chang, Yuehong,Liu, Jiandong,Liu, Huifang,Liu, Di,Kong, Xiangang.

[3]Molecular and biochemical characterization of a distinct tyrosinase involved in melanin production from Aeromonas media. Wan, Xia,Chai, Baozhong,Liao, Yi,Ye, Tao,Shen, Ping,Chen, Xiangdong,Wan, Xia,Su, Ying.

[4]Codon optimization of Bacillus licheniformis beta-1,3-1,4-glucanase gene and its expression in Pichia pastoris. Teng, Da,Fan, Ying,Yang, Ya-lin,Tian, Zi-gang,Luo, Jin,Wang, Jian-hua.

[5]Eukaryotic expression and antimicrobial spectrum determination of the peptide tachyplesin II. Xu, Feng,Meng, Kun,Wang, Ya-Ru,Lu, Hui-Ying,Yang, Pei-Long,Yao, Bin,Xu, Feng,Wu, Ning-Feng,Fan, Yun-Liu.

[6]Efficient recovery of the functional IP10-scFv fusion protein from inclusion bodies with an on-column refolding system. Guo, JQ,Li, QM,Zhou, JY,Zhang, GP,Yang, YY,Xing, GX,Zhao, D,You, SY,Zhang, CY.

[7]The ecdysteroid UDP-glucosyltransferase gene promoter from Autographa californica multicapsid nucleopolyhedrovirus. Shen, XJ,Yi, YZ,Tang, SM,Zhang, ZF,Li, YR,He, JL.

[8]Genome-wide analysis of SINA family in plants and their phylogenetic relationships. Wang, Meng,Jin, Ying,Fu, Junjie,Zhu, Yun,Hu, Jian,Wang, Guoying,Zheng, Jun,Wang, Guoying.

[9]Characterization of FAE1 in the zero erucic acid germplasm of Brassica rapa L.. Yan, Guixin,Li, Dan,Cai, Mengxian,Gao, Guizhen,Chen, Biyun,Xu, Kun,Li, Jun,Li, Feng,Wang, Nian,Qiao, Jiangwei,Li, Hao,Zhang, Tianyao,Wu, Xiaoming.

[10]Effects of kanamycin on tissue culture and somatic embryogenesis in cotton. Bao-Hong Zhang,Fang Liu,Zhi-Hong Liu,Hong-Mei Wang,Chang-Bing Yao.

[11]Cloning and characterization of a novel Cry1A toxin from Bacillus thuringiensis with high toxicity to the Asian corn borer and other lepidopteran insects. Liang, Gemei,Li, Haitao,He, Kanglai,Song, Fuping,Feng, Xue,Zhang, Jie,Crickmore, Neil,Huang, Dafang.

[12]Engineered Bacillus thuringiensis GO33A with broad insecticidal activity against lepidopteran and coleopteran pests. Wang, Guangjun,Zhang, Jie,Song, Fuping,Wu, Jun,Feng, Shuliang,Huang, Dafang.

[13]Molecular cloning and characterization of a mannose-binding lectin gene from Crinum asiaticum. Pang, YZ,Liao, ZH,Zhang, L,Sun, XF,Lu, YQ,Wang, SG,Tang, KX.

[14]Mining, identification and function analysis of microRNAs and target genes in peanut (Arachis hypogaea L.). Zhang, Tingting,Hu, Shuhao,Yan, Caixia,Li, Chunjuan,Zhao, Xiaobo,Shan, Shihua,Hu, Shuhao,Wan, Shubo.

[15]Antifungal activity of Penicillium striatisporum Pst10 and its biocontrol effect on Phytophthora root rot of chilli pepper. Ma, Yan,Zhou, Ming-guo,Ma, Yan,Chang, Zhi-zhou,Zhao, Jiang-tao.

[16]Cry8Ca2-containing layer-by-layer microcapsules for the pH-controlled release of crystal protein. Yan, Yue,Wang, Dandan,Guo, Shuyuan,Zhang, Jie. 2014

[17]Reduction of Bacillus thuringiensis Cry1Ac toxicity against Helicoverpa armigera by a soluble toxin-binding cadherin fragment. Liu, Chenxi,Wu, Kongming,Gao, Yulin,Ning, Changming,Wu, Yidong,Ning, Changming,Oppert, Brenda.

[18]Photorhabdus luminescens toxin-induced permeability change in Manduca sexta and Tenebrio molitor midgut brush border membrane and in unilamellar phospholipid vesicle. Liu, W,Ye, WH,Wang, ZM,Wang, XR,Tian, SY,Cao, HL,Lian, JY.

[19]Co-ordinate expression of glycine betaine synthesis genes linked by the FMDV 2A region in a single open reading frame in Pichia pastoris. Wang, Sanhong,Yao, Quanhong,Tao, Jianmin,Qiao, Yushan,Zhang, Zhen.

[20]Isolation and characterization of induced genes under drought stress at the flowering stage in maize (Zea mays). Li, Hui-Yong,Wang, Tian-Yu,Shi, Yun-Su,Fu, Jun-Jie,Song, Yan-Chun,Wang, Guo-Ying,Li, Yu.

作者其他论文 更多>>