Fluorescent differential display analysis of gene expression for NPV resistance in Bombyx mori L.

文献类型： 外文期刊

第一作者： Xu, JP

作者： Xu, JP;Chen, KP;Yao, Q;Liu, XY

作者机构：

关键词： BmNPV;FDD;resistance

期刊名称：JOURNAL OF APPLIED ENTOMOLOGY （ 影响因子：2.603； 五年影响因子：2.562 ）

ISSN： 0931-2048

年卷期： 2005 年 129 卷 1 期

页码：

收录情况： SCI

摘要： Using the fluorescent differential display (FDD) technique, we analysed the differential expression of genes related to BmNPV ( Bombyx mori nuclear polyhedrosis virus) resistance. Silkworm strains used included a highly resistant strain named NB, a highly susceptible strain named 306 and a near-isogenic line named 306NNZZ. Two differential bands, G12(532) and G13(313), were found linked to BmNPV resistance and were further identified. Band G12(532) was found in the midguts of the eighth generation of backcross (BC(8)) of strain 306NNZZ but was not present in those of strain 306. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis confirmed that the band existed in BC8 of strains 306NNZZ and NB while not in strain 306. Northern blot analysis showed that G12(532) expressed actively in BC8 of strains 306NNZZ and NB while expressed inactively in strain 306. These results suggest that the G12(532) is a fragment of the gene related to silkworm's resistance to BmNPV disease. The sequence of G12(532) has an 85% similarity to sequences with GenBank accession numbers of AV398077 and AV398034 that are relative to BmNPV infection demonstrated by previous studies. Its coding region ( 37 amino acids) has 54% similarity with B. mori endonuclease and reverse transcriptase-like protein. Band G13(313) was appeared in the hemolymph of highly resistant strain NB and near isogenic line 306NNZZ in FDD, but was not found in highly susceptible strain 306, all of which were confirmed by RT-PCR and Northern blot analyses. All the experimental results obtained suggest that G13(313) is also a fragment of the gene related to BmNPV resistance. The sequence of band G13(313) does not have a high similarity to the above relative sequences in GenBank. It might be a new RNA fragment related to NPV resistance. Our study also showed that the FDD technique could be a promising approach in identifying novel genes while appropriate silkworm strains are used.

分类号：

  • 相关文献

[1]Identification and characterization of Bms3a in Bombyx mori L.. Chen Ke-Ping,Yao Qin,Gao Gui-Tian,Zhao Yuan,Xu Jia-Ping,Liu Ming-Hui. 2008

[2]Comparison of factors that may affect the inhibitory efficacy of transgenic RNAi targeting of baculoviral genes in silkworm, Bombyx mori. Jiang, Liang,Zhao, Ping,Wang, Genhong,Cheng, Tingcai,Jin, Shengkai,Lin, Ping,Xia, Qingyou,Jiang, Liang,Zhao, Ping,Wang, Genhong,Cheng, Tingcai,Jin, Shengkai,Lin, Ping,Sun, Qiang,Xia, Qingyou,Yang, Qiong,Xiao, Yang.

[3]Identification and characterization of an NPV infection-related gene Bmsop2 in Bombyx mori L.. Xu, JP,Chen, KP,Yao, Q,Liu, MH,Gao, GT,Zhao, Y. 2005

[4]Effects of cetyltriethylammonium bromide on the replication of Bombyx mori nucleopolyhedrovirus. Zhou, YJ,Zhang, ZF,He, JL,Zhang, YX.

[5]BmNPV-miR-415 up-regulates the expression of TOR2 via Bmo-miR-5738. Cao, Xueliang,Xia, Dingguo,Qiu, Zhiyong,Shen, Xingjia,Guo, Xijie,Zhao, Qiaoling,Huang, Yong,Cao, Xueliang,Huang, Yong,Xia, Dingguo,Qiu, Zhiyong,Shen, Xingjia,Guo, Xijie,Zhao, Qiaoling. 2017

[6]Entry of Bombyx mori nucleopolyhedrovirus into BmN cells by cholesterol-dependent macropinocytic endocytosis. Huang, Jinshan,Hao, Bifang,Cheng, Chen,Liang, Fei,Shen, Xingjia,Huang, Jinshan,Hao, Bifang,Shen, Xingjia,Cheng, Xiaowen.

[7]Gene expression profiling of resistant and susceptible Bombyx mori strains reveals nucleopolyhedrovirus-associated variations in host gene transcript levels. Bao, Yan-Yuan,Tang, Xu-Dong,Lv, Zu-Yao,Tian, Cai-Hong,Xu, Yi Peng,Zhang, Chuan-Xi,Wang, Xiu-Ye. 2009

[8]The mutation in nicotinic acetylcholine receptor beta 1 subunit may confer resistance to imidacloprid in Aphis gossypii (Glover). Shi, Xu-Gen,Zhu, Yu-Kun,Xia, Xiao-Ming,Qiao, Kang,Wang, Kai-Yun,Wang, Hong-Yan. 2012

[9]Fine mapping of the tomato yellow leaf curl virus resistance gene Ty-2 on chromosome 11 of tomato. Yang, Xiaohui,Guo, Yanmei,Wang, Xiaoxuan,Du, Yongchen,Yang, Xiaohui,Yang, Xiaohui,Hutton, Samuel F.,Scott, John W.,Caro, Myluska,Rashid, Md Harunur,Visser, Richard G. F.,Bai, Yuling,Szinay, Dora,de Jong, Hans. 2014

[10]Resistance of Helicoverpa assulta (Guenee) (Lepidoptera: Noctuidae) to fenvalerate, phoxim and methomyl in China. Wang, Kai Yun,Xia, Xiao Ming,Wang, Hong Yan. 2009

[11]Ecological fitness of fludioxonil-resistant Rhizoctonia cerealis strain and its cross-resistance to DMIs and validamycin. Xia, Xiaoming,Zhao, Ming,Wang, Hongyan,Ma, Hui,Wang, Kaiyun. 2012

[12]Effect of pyramiding Bt and CpTI genes on resistance of cotton to Helicoverpa armigera (Lepidoptera: Noctuidae) under laboratory and field conditions. Jinjie Cui,Junyu Luo,Wopke Van Der Werf,Yan Ma,Jingyuan Xia.

[13]Effects of kanamycin on tissue culture and somatic embryogenesis in cotton. Bao-Hong Zhang,Fang Liu,Zhi-Hong Liu,Hong-Mei Wang,Chang-Bing Yao.

[14]Antimicrobial resistance in Escherichia coli isolates from food animals, animal food products and companion animals in China. Lei, Tao,Tian, Wei,He, Liu,Huang, Xian-Hui,Sun, Yong-Xue,Deng, Yu-Ting,Sun, Yan,Liu, Jian-Hua,Lv, Dian-Hong,Wu, Cong-Ming,Shen, Jian-Zhong,Huang, Liang-Zong. 2010

[15]RNA-Seq Analyses for Two Silkworm Strains Reveals Insight into Their Susceptibility and Resistance to Beauveria bassiana Infection. Xing, Dongxu,Jiang, Liang,Xia, Qingyou,Xing, Dongxu,Yang, Qiong,Li, Qingrong,Xiao, Yang,Ye, Mingqiang. 2017

[16]Determination of baseline susceptibility to Cry1Ab protein for Asian corn borer (Lep., Crambidae). Wang, Z,Wen, L,Bai, S,Ma, X,Yao, Z. 2005

[17]Resistance Performances of Transgenic Bt Rice Lines T-2A-1 and T1c-19 Against Cnaphalocrocis medinalis (Lepidoptera: Pyralidae). Zheng, Xusong,Yang, Yajun,Xu, Hongxing,Wang, Baoju,Lu, Zhongxian,Zheng, Xusong,Yang, Yajun,Xu, Hongxing,Wang, Baoju,Lu, Zhongxian,Chen, Hao,Lin, Yongjun,Chen, Hao,Lin, Yongjun. 2011

[18]Analysis of Extreme Phenotype Bulk Copy Number Variation (XP-CNV) Identified the Association of rp1 with Resistance to Goss's Wilt of Maize. Hu, Ying,Ren, Jie,Umana, Arnoldo A.,Le, Ha,Danilova, Tatiana,Liu, Sanzhen,Peng, Zhao,White, Frank F.,Fu, Junjie,Wang, Haiyan,Robertson, Alison,Hulbert, Scot H.,Umana, Arnoldo A.. 2018

[19]Two Lysin-Motif Receptor Kinases, Gh-LYK1 and Gh-LYK2, Contribute to Resistance against Verticillium wilt in Upland Cotton. Li, Fangfang,Qian, Shasha,Zhou, Xueping,Gu, Zhouhang,Wang, Qian,Ye, Fei,Liu, Tingli,Chen, Tianzi,Yang, Yuwen,Wang, Jinyan,Zhang, Baolong,Ding, Bo,Wang, Guoliang,Zhou, Xueping. 2017

[20]A review of sublethal effects of pulsed electric field on cells in food processing. Wang, Man-Sheng,Yang, Jing,Hou, Zhen-Ping,Wang, Yan-Zhou,Dai, Qiu-Zhong,Wang, Lang-Hong,Zeng, Xin-An,Bekhit, Alaa El-Din A.. 2018

作者其他论文 更多>>

微信小程序