Identification and characterization of an NPV infection-related gene Bmsop2 in Bombyx mori L.
文献类型: 外文期刊
第一作者: Xu, JP
作者: Xu, JP;Chen, KP;Yao, Q;Liu, MH;Gao, GT;Zhao, Y
作者机构:
关键词: Bombyx mori;Bmsop2;FDD;NPV;susceptibility
期刊名称:JOURNAL OF APPLIED ENTOMOLOGY ( 影响因子:2.603; 五年影响因子:2.562 )
ISSN: 0931-2048
年卷期: 2005 年 129 卷 8 期
页码:
收录情况: SCI
摘要: Using the fluorescent differential display technique, we analysed the differential expression of genes related to Bombyx mori nuclear polyhedrosis virus ( BmNPV) resistance. Silkworm strains studied included the highly resistant strain NB, highly susceptible strain 306 and near-isogenic line 306NNZZ. One novel gene was identified and named Bmsop2 for its high similarity with the Sop2 protein of other species. It was identified to be linked to BmNPV susceptibility by Northern blotting and real-time polymerase chain reaction. The results indicated that it was actively expressed in midguts of strains 306, NB and the eighth generation of backcross (BC8) of strain 306NNZZ which had been treated with BmNPV. But the expression level was low in the midguts of the control. In the mean time, the expression of Bmsop2 was the highest in strain 306 treated with BmNPV while it was the lowest in strain 306 not treated with BmNPV. Our study showed that Bmsop2 is a differentially expressed gene in strains NB, 306 and 306NNZZ which have different levels of resistance to BmNPV.
分类号:
- 相关文献
作者其他论文 更多>>
-
Fluorescent differential display analysis of gene expression for NPV resistance in Bombyx mori L.
作者:Xu, JP;Chen, KP;Yao, Q;Liu, XY
关键词:BmNPV;FDD;resistance
-
A pokeweed antiviral protein gene in roots of Phytolacca americana
作者:Zhao, Y;Wang, X;Zhou, G;Li, H
关键词:
-
Transgene expression in strawberries driven by a heterologous phloem-specific promoter
作者:Zhao, Y;Liu, QZ;Davis, RE
关键词:strawberry;AtSUC2-promoter;indirect regeneration;phloem-specific expression
-
Screening of molecular markers for NPV resistance in Bombyx mori L. (Lep., Bombycidae)
作者:Yao, Q;Li, MW;Wang, Y;Wang, WB;Lu, J;Dong, Y;Chen, KP
关键词:
-
Small subunit ribosomal RNA genes of microsporidia
作者:Wang, JY;Huang, KW;Mao, XC;Zhao, Y;Lu, CD
关键词:microsporidia;ssurRNA gene;phylogenetic relationship
-
Molecular cytogenetic analysis of a durum wheat x Thinopyrum distichum hybrid used as a new source of resistance to Fusarium head blight in the greenhouse
作者:Chen, Q;Eudes, F;Conner, RL;Graf, R;Comeau, A;Collin, J;Ahmad, F;Zhou, R;Li, H;Zhao, Y;Laroche, A
关键词:Triticum durum;Thinopyrum distichum;Fusarium graminearum;Fusarium head blight;alien hybrids;FHB resistance;genetic resources;chromosomal introgression;GISH-pattern
-
Altering fibroin heavy chain gene of silkworm Bombyx mori by homologous recombination
作者:Zhao, Y;Chen, X;Peng, WP;Dong, L;Huang, JT;Lu, CD
关键词:silkworm (Bombyx mori);GFP;synthetic fibroin-like gene;transgenic;homologous recombination