Transgene expression in strawberries driven by a heterologous phloem-specific promoter

文献类型： 外文期刊

第一作者： Zhao, Y

作者： Zhao, Y;Liu, QZ;Davis, RE

作者机构：

关键词： strawberry;AtSUC2-promoter;indirect regeneration;phloem-specific expression

期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

ISSN： 0721-7714

年卷期： 2004 年 23 卷 4 期

页码：

收录情况： SCI

摘要： Strawberry is susceptible to diseases caused by phytoplasmas, mycoplasma-like prokaryotes restricted to sieve elements in the phloem tissue of infected plants. One strategy to improve strawberry resistance to phytoplasmas involves transgenic expression of anti-microbial peptide genes in phloem. For targeted phloem-specific expression, we constructed a binary vector with an expression cassette bearing the beta-glucuronidase (GUS) reporter gene (uidA) under control of the Arabidopsis sucrose-H+ symporter gene (AtSUC2) promoter. Transgenic strawberry lines were generated with high efficiencies by a modified transformation protocol, which combines the adoption of a 3-day pre-selection period following transformation, and the addition of 10-muM thidiazuron to the regeneration medium. Histological GUS activity indicated that the reporter gene was expressed specifically in phloem of leaves, petioles, and roots of transgenic plants. The results suggest that the transformation protocol and the AtSUC2 promoter may be useful for engineering phytoplasma-resistant transgenic strawberries.

分类号：

  • 相关文献

[1]Evaluation of Chloropicrin Gelatin Capsule Formulation as a Soil Fumigant for Greenhouse Strawberry in China. Yan, Dongdong,Wang, Qiuxia,Mao, Liangang,Xie, Hongwei,Guo, Meixia,Cao, Aocheng.

[2]Characterization of the Hormone and Stress-Induced Expression of FaRE1 Retrotransposon Promoter in Strawberry. He, Ping,Ma, Yue,Dai, Hongyan,Liu, Yuexue,Li, He,Zhang, Zhihong,He, Ping,Li, Linguang,Zhao, Guiling.

[3]Highly Effective Cultivation Techniques for Interplanting Strawberry and Melon. Cao Guishou,Wang Junyu.

[4]Effect of BTH on antioxidant enzymes, radical-scavenging activity and decay in strawberry fruit. Cao, Shifeng,Hu, Zhichao,Lu, Binhong,Zheng, Yonghua,Yang, Zhenfeng.

[5]A non-destructive method to predict polyphenol content in strawberry. Fan, Li,Dube, Claudine,Tremblay, Nicolas,Khanizadeh, Shahrokh,Fan, Li,Fang, Chengquan.

[6]Dissipation of four fungicides on greenhouse strawberries and an assessment of their risks. Wang, Zhiwei,Cang, Tao,Qi, Peipei,Zhao, Xuepin,Xu, Hao,Wang, Xiangyun,Zhang, Hu,Wang, Xinquan.

[7]Lanthanum nitrate regulates the content of vitamin C through its biosynthesis, regeneration and degradation in the fruit of strawberry. Shan, Changjuan,Zhou, Houcheng,Shan, Changjuan,Zhang, Huixi,Zhang, Yongyong,Zhou, Houcheng,Zhou, Houcheng.

[8]Strawberry Breeding in Zhejiang of China. Jiang, G. H.,Zhang, Y. C.,Miao, L. X.,Yang, X. F..

[9]Isolation and characterization of the carotenoid biosynthetic genes LCYB, LCYE and CHXB from strawberry and their relation to carotenoid accumulation. Zhu, Haisheng,Chen, Mindong,Wen, Qingfang,Li, Yongping,Zhu, Haisheng,Chen, Mindong,Wen, Qingfang,Li, Yongping.

[10]Technology of Anthracnose Control on Increasing Strawberry Plantlets. Jiang, G. H.,Miao, L. M.,Yang, X. F.,Zhang, Y. C.,Ren, H. Y.,Wang, H. R..

[11]Residue Analysis and Degradation Studies of Fenbuconazole and Myclobutanil in Strawberry by Chiral High-Performance Liquid Chromatography-Tandem Mass Spectrometry. Zhang, Hu,Wang, Xinquan,Qian, Mingrong,Wang, Xiangyun,Xu, Hao,Xu, Mingfei,Wang, Qiang.

[12]Efficient Identification of 46 Important Strawberry Cultivars Using RAPD Markers with a Manual Cultivar Identification Diagram Strategy. Zhao, Mizhen,Wang, Jing,Wang, Yujuan,Kayesh, E.,Song, Changnian,Dong, Qinghua,Li, Jinfeng.

[13]Oxygen Radical Absorbance Capacity of Different Varieties of Strawberry and the Antioxidant Stability in Storage. Li, Chunyang,Huang, Wu-Yang,Wang, Xing-Na,Liu, Wen-Xu,Liu, Wen-Xu.

[14]Colored light-quality selective plastic films affect anthocyanin content, enzyme activities, and the expression of flavonoid genes in strawberry (Fragaria x ananassa) fruit. Miao, Lixiang,Zhang, Yuchao,Yang, Xiaofang,Xiao, Jinping,Zhang, Huiqin,Zhang, Zuofa,Wang, Yuezhi,Jiang, Guihua.

[15]Analysis of Tebuconazole and Tetraconazole Enantiomers by Chiral HPLC-MS/MS and Application to Measure Enantioselective Degradation in Strawberries. Zhang, Hu,Wang, Minghua,Zhang, Hu,Qian, Mingrong,Wang, Xinquan,Wang, Xiangyun,Xu, Hao,Qi, Peipei,Wang, Qiang.

[16]PHENOLIC COMPOSITION AND ANTIOXIDANT CAPACITY OF NEWLY DEVELOPED STRAWBERRY LINES FROM BRITISH COLUMBIA AND QUEBEC. Ranieri, Marta,Khanizadeh, Shahrokh,Yu, Cui,Lv, Deguo,Yu, Cui,Ranieri, Marta,Zhang, Maojun,Charles, Marie Therese,Rekika, Djamila,Khanizadeh, Shahrokh,Zhang, Maojun,Tsao, Rong.

[17]'Ningyu': a New Cultivar with High Reducing Sugar, Ascorbic Acid and Antioxidant Capacity. Wang, J.,Zhao, M. Z.,Wang, Z. W.,Yu, H. M.,Huang, W. Y..

[18]Studies on Stereoscopic Cultivation in Strawberry. Zhang, Y. C.,Miao, L. X.,Yang, X. F.,Jiang, G. H..

[19]Effect of rain shelter and shading on plantlets growth and antioxidant contents in strawberry. Miao, Lixiang,Zhang, Yuchao,Yang, Xiaofang,Jiang, Guihua.

[20]Evaluation of Sporidiobolus pararoseus strain YCXT3 as biocontrol agent of Botrytis cinerea on post-harvest strawberry fruits. Huang, R.,Che, H. J.,Zhang, J.,Yang, L.,Jiang, D. H.,Li, G. Q.,Huang, R.,Che, H. J.,Zhang, J.,Yang, L.,Jiang, D. H.,Li, G. Q.,Huang, R..

作者其他论文 更多>>

微信小程序