Transformation of neomycin resistance gene (neo(R)) into silkworm (Bombyx mori L.)

文献类型： 外文期刊

第一作者： Chen, X

作者： Chen, X;Zhao, Y;Zhang, F;Peng, WP;Feng, XL;Huang, JT;Lu, CD

作者机构：

关键词： Bombyx mori. L.;selective marker;neo(R);transgenic silkworm

期刊名称：ACTA BIOCHIMICA ET BIOPHYSICA SINICA （ 影响因子：3.848； 五年影响因子：3.67 ）

ISSN： 0582-9879

年卷期： 1999 年 31 卷 1 期

页码：

收录情况： SCI

摘要： Neomycin resistance gene (neo(R)) flanked by 5' and 3' regions of fibroin H-chain gene of silkworm (Bombyx mori. L.) was transferred into eggs of silkworm by gene gun in the early period of fertilization. The larvae were fed with an artificial diet containing neomycin in early 24 hours post transfettion, and some of them survived. The neo(R) encoding sequence in G(2) generation derived from the survivals was detected by Southern blotting. The results indicated that neo(R) could be used as a selective marker for studies on transgenic silk-worm.

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