A ligation-driven CRISPR-Cas biosensing platform for non-nucleic acid target detections

文献类型： 外文期刊

第一作者： Zhao, Jiali

作者： Zhao, Jiali;Tan, Zhen;Lei, Chunyang;Nie, Zhou;Wang, Liu

作者机构：

期刊名称：CHEMICAL COMMUNICATIONS （ 影响因子：6.222； 五年影响因子：6.008 ）

ISSN： 1359-7345

年卷期： 2021 年 57 卷 57 期

页码：

收录情况： SCI

摘要： Herein, we describe a CRISPR-Cas12a sensing platform activated by a DNA ligation reaction for the sensitive detection of non-nucleic acid targets, including NAD(+), ATP and polynucleotide kinase (PNK). In this design, the DNA ligation reaction triggered by these biomolecules generates DNA duplexes, which can activate the nuclease activity of Cas12a to produce amplified fluorescence signals. As a result, this work provides an alternative strategy to expand the applicability of the CRISPR-Cas system into the detection of non-nucleic acid biomolecules.

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