High-through counting of Chinese cabbage trichomes based on deep learning and trinocular stereo microscope

文献类型： 外文期刊

第一作者： Li, Xiyao

作者： Li, Xiyao;He, Yong;Yang, Guofeng;Tao, Yimin;Feng, Xuping;Chen, Jingwen;Li, Zhongren;Huang, Li;Li, Yanda;Li, Yu

作者机构：

关键词： Object detection; Counting; Germplasm resources; Trichomes; Chinese cabbage

期刊名称：COMPUTERS AND ELECTRONICS IN AGRICULTURE （ 影响因子：8.3； 五年影响因子：8.3 ）

ISSN： 0168-1699

年卷期： 2023 年 212 卷

页码：

收录情况： SCI

摘要： The trichome trait is one of the important phenotypes for variety classification and breeding improvement of Chinese cabbage (Brassica campestris L. syn. B. rapa). However, obtaining the number of trichomes per unit area on leaves is a time-consuming and laborious detection work, especially when hundreds of germplasm resources need to be evaluated. Therefore, this study constructed the first diverse Chinese cabbage trichome dataset called CCTD with10,955 RGB images and proposed a deep learning model for trichome detection called TRI-YOLOv8. By adding the RepVGG module in the Backbone, adding a new detection layer in the Neck and replacing the loss function with Normalized Gaussian Wasserstein Distance Loss, the detection performance of the model for small trichomes was effectively improved. At the same time, Ghost convolution was used to reduce memory consumption and speed up inference. The experimental results showed that TRI-YOLOv8 outperformed other classical detection models. AP50 was as high as 94.4%, which was 3.8% higher than YOLOv8n. Furthermore, the number of trichomes per unit area was obtained by TRI-YOLOv8 and combined with genome-wide association study and selective sweep analysis, the candidate gene BraA03g029740.3.5C (STP7) was screened out. Overall, this study achieved the accurate detection and counting of trichomes, and provided a feasible plan for breeders to digitally analyze phenotypes, automatically identify and screen Chinese cabbage germplasm resources.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序