The tissue virome of black-spotted frogs reveals a diversity of uncharacterized viruses

文献类型： 外文期刊

第一作者： Li, Chenxi

作者： Li, Chenxi;Liu, Yuhang;Li, Nan;Yi, Le;Tu, Changchun;He, Biao;Hu, Yazhou;Tu, Changchun

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关键词： virome; black-spotted frogs; genetic diversity; tissue tropism

期刊名称：VIRUS EVOLUTION （ 影响因子：5.5； 五年影响因子：6.1 ）

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年卷期： 2024 年 10 卷 1 期

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收录情况： SCI

摘要： Amphibians are an essential class in the maintenance of global ecosystem equilibrium, but they face serious extinction risks driven by climate change and infectious diseases. Unfortunately, the virus diversity harbored by these creatures has been rarely investigated. By profiling the virus flora residing in different tissues of 100 farmed black-spotted frogs (Rana nigromaculata) using a combination of DNA and RNA viromic methods, we captured 28 high-quality viral sequences covering at least 11 viral families. Most of these sequences were remarkably divergent, adding at least 10 new species and 4 new genera within the families Orthomyxoviridae, Adenoviridae, Nodaviridae, Phenuiviridae, and Picornaviridae. We recovered five orthomyxovirus segments, with three distantly neighboring two Chinese fish-related viruses. The recombination event of frog virus 3 occurred among the frog and turtle strains. The relative abundance and molecular detection revealed different tissue tropisms of these viruses, with the orthomyxovirus and adenoviruses being enteric and probably also neurotropic, but the new astrovirus and picornavirus being hepatophilic. These results expand the spectrum of viruses harbored by anurans, highlighting the necessity to continuously monitor these viruses and to investigate the virus diversity in a broader area with more diverse amphibian species.

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