An ultrasensitive, rapid and portable method for screening oseltamivir-resistant virus based on CRISPR/Cas12a combined with immunochromatographic strips

文献类型： 外文期刊

第一作者： Zhang, Hao

作者： Zhang, Hao;Yang, Fayu;Yang, Man;Liu, Jing;Wang, Mi;Fei, Chenzhong;Zhang, Lifang;Xue, Feiqun;Zhu, Chuangang;Liu, Yingchun;Gu, Feng;Zhang, Hao;Yang, Fayu;Liu, Jing;Wang, Mi;Fei, Chenzhong;Zhang, Lifang;Xue, Feiqun;Liu, Yingchun;Gu, Feng;Yang, Man;Zhu, Chuangang

作者机构：

关键词： influenza virus; CRISPR/Cas12a; sensitivity; lateral flow detection

期刊名称：ACTA BIOCHIMICA ET BIOPHYSICA SINICA （ 影响因子：3.7； 五年影响因子：3.9 ）

ISSN： 1672-9145

年卷期： 2022 年 54 卷 11 期

页码：

收录情况： SCI

摘要： Influenza is a significant public health challenge because of the emergence of antigenically shifted or highly virulent strains. The neuraminidase inhibitor oseltamivir is used as an antiviral drug in clinical treatment. However, its therapeutic effects can be greatly compromised by the emergence of drug-resistant mutant viruses. Thus, there is an urgent need to distinguish drug-resistant strains with a simple method. To address this, in the present study, we develop a rapid, sensitive and convenient molecular diagnosis method based on CRISPR/Cas12a technology and lateral flow detection (LFD). By targeting mutant sequences amplified by recombinase polymerase amplification (RPA) reaction, crRNA is designed to develop the CRISPR/Cas12a assay, and 2000 copies can be directly observed by the naked eye under blue light-emitting diode (LED) light. Combined with LFD, the limit of detection of RPA-CRISPR/Cas12a-LFD is about 20 copies of target sequence per reaction. Collectively, RPA-CRISPR/Cas12a-LFD method provides a novel alternative for the sensitive, specific and portable detection to diagnose oseltamivir-resistant mutant strains.

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