Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation

文献类型： 外文期刊

第一作者： Tao, Xiaoyuan

作者： Tao, Xiaoyuan;Wang, Siyuan;Guan, Xueying;Tao, Xiaoyuan;Gao, Mengtao;Guan, Xueying

作者机构：

期刊名称：JOVE-JOURNAL OF VISUALIZED EXPERIMENTS （ 影响因子：1.424； 五年影响因子：1.789 ）

ISSN： 1940-087X

年卷期： 2022 年 182 期

页码：

收录情况： SCI

摘要： Epigenomic regulation at the chromatic level, including DNA and histone modifications, behaviors of transcription factors, and non-coding RNAs with their recruited proteins, lead to temporal and spatial control of gene expression. Cleavage under targets and tagmentation (CUT&Tag) is an enzyme-tethering method in which the specific chromatin protein is firstly recognized by its specific antibody, and then the antibody tethers a protein A-transposase (pA-Tn5) fusion protein, which cleaves the targeted chromatin in situ by the activation of magnesium ions. Here, we provide our previously published CUT&Tag protocol using intact nuclei isolated from allortetraploid cotton leaves with modification. This step-by-step protocol can be used for epigenomic research in plants. In addition, substantial modifications for plant nuclei isolation are provided with critical comments.

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