Effects of cetyltriethylammonium bromide on the replication of Bombyx mori nucleopolyhedrovirus

文献类型： 外文期刊

第一作者： Zhou, YJ

作者： Zhou, YJ;Zhang, ZF;He, JL;Zhang, YX

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关键词： Bmnpv;Ctab;Infection;Insect cells;Silkworm larvae;Transfection;Nuclear polyhedrosis-virus;Immediate-early gene;Ni granulosis-virus;Nucleotide-sequence;Trichoplusia-ni;Peritrophic membrane;Expression vectors;Dna-replication;Insect cells;Baculovirus

期刊名称：CYTOTECHNOLOGY （ 影响因子：2.058； 五年影响因子：2.362 ）

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收录情况： SCI

摘要： An experimental study was undertaken to quantify the effects of cetyltriethylammonium bromide (CTAB) on the replication of Bombyx mori nucleopolyhedrovirus (BmNPV) and the transcriptional activity of BmNPV ie-1 promoter. The results demonstrated that the budded virus (BV) titer rose about 3.7-fold by adding CTAB to the culture media up to 0.1 mug ml(-1) in infected Bm-N cells with a wild-type BmNPV. The transient expression level of luciferase driven by BmNPV ie-1 promoter was enhanced by more than 3-fold in the presence of 0.1 mug ml(-1) of CTAB in uninfected insect cells via a transient expression system. Contrary to the rise in BV titer, the polyhedra inside the nucleus of infected cells dropped linearly from 4.0 X 10(6) ml(-1) down to 2.1 X 10(6) ml(-1) within a range of CTAB concentrations from 0 to 0.25 mug ml(-1). The same trend in expression level of beta-galactosidase or phytase was given when the Bm-N cells or fifth-instar silkworm larvae infected with a recombinant BmNPV containing the beta-galactosidase or phytase reporter gene driven by the polyhedrin promoter. We deduced that CTAB appeared to affect the virus bi-phasic life cycle stages and production pathways, resulting in an enhancement in BV production and a suppression of occluded virus (OV) production and expression of foreign genes controlled by the polyhedrin promoter. [References: 43]

分类号： Q2

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