Isolation, characterization and whole genome analysis of the novel genus Lederbergvirus, phage vB_EcoP_E212 infecting enterotoxigenic Escherichia coli K88

文献类型： 外文期刊

第一作者： Wei, Bingdong

作者： Wei, Bingdong;Zheng, Lin;Chen, Long;Yan, Xiaogang;Cong, Cong

作者机构：

关键词： Enterotoxigenic Escherichia coli; K88; Phage; Genome analysis; Biological characteristics

期刊名称：VIRUS RESEARCH （ 影响因子：5.0； 五年影响因子：4.0 ）

ISSN： 0168-1702

年卷期： 2023 年 331 卷

页码：

收录情况： SCI

摘要： The newly discovered phage vB_EcoP_E212 (also known as E212) was characterized and its genome was annotated in this study, which was conducted in Jilin, China. Transmission electron microscopy indicates that phage E212 belongs to the class Caudoviricetes. This phage exclusively infects enterotoxigenic E. coli K88. E212 was found to have a short latent period of 20 min, and a burst size of 125 PFU/cell. Additionally, E212 remained stable at all pH levels (3.0-12.0) and temperatures between -20 and 60 oC. The genome of the phage E212 consists of 38,252 bp dsDNA molecule with a G + C content of 46.98%. The genome is projected to include 53 ORFs but no tRNAs. This phage lacks homologs of virulence factors or antimicrobial resistance genes, but it has lysogeny-related genes. Phage E212 was placed in the genus Lederbergvirus as a result of nucleotide sequence alignment and phylogenetic analysis.

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