Exploring Multi-Tissue Alternative Splicing and Skeletal Muscle Metabolism Regulation in Obese- and Lean-Type Pigs

文献类型： 外文期刊

第一作者： Wang, Wei

作者： Wang, Wei;Tang, Zhonglin;Wang, Wei;Tang, Zhonglin;Wang, Wei;Li, Wangchang;Liu, Weiwei;Wang, Zishuai;Tang, Zhonglin;Li, Wangchang;Liu, Weiwei;Yang, Xiaogan;Tang, Zhonglin;Li, Wangchang;Liu, Weiwei;Wang, Zishuai;Tang, Zhonglin;Xie, Bingkun;Tang, Zhonglin

作者机构：

关键词： obese- and lean-type pigs; multiple tissues; transcriptome; alternative splicing; skeletal muscle metabolism

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

ISSN：

年卷期： 2024 年 15 卷 2 期

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收录情况： SCI

摘要： Alternative splicing (AS) is a crucial mechanism in post-transcriptional regulation, contributing significantly to the diversity of the transcriptome and proteome. In this study, we performed a comprehensive AS profile in nine tissues obtained from Duroc (lean-type) and Luchuan (obese-type) pigs. Notably, 94,990 AS events from 14,393 genes were identified. Among these AS events, it was observed that 80% belonged to the skipped exon (SE) type. Functional enrichment analysis showed that genes with more than ten AS events were closely associated with tissue-specific functions. Additionally, the analysis of overlap between differentially alternative splicing genes (DSGs) and differentially expressed genes (DEGs) revealed the highest number of overlapped genes in the heart and skeletal muscle. The novelty of our study is that it identified and validated three genes (PYGM, MAPK11 and CAMK2B) in the glucagon signaling pathway, and their alternative splicing differences were highly significant across two pig breeds. In conclusion, our study offers novel insights into the molecular regulation of diverse tissue physiologies and the phenotypic differences between obese- and lean-type pigs, which are helpful for pig breeding.

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