YM-1: A novel deoxynivalenol-detoxifying bacterial consortium from intestines of free-range chickens

文献类型： 外文期刊

第一作者： Wu, Yiming

作者： Wu, Yiming;Zhao, Chongqi;Song, Guanzhong;Shen, Haibo;Ma, Xiaokang;Tan, Bie;Yin, Yulong;Jiang, Qian;Li, Xilong;Jiang, Qian;Yin, Yulong

作者机构：

关键词： Deoxynivalenol; De-epoxidize; IPEC-J2 cells; Cytotoxicity; DOM-1; Chicken intestines

期刊名称：ANNALS OF AGRICULTURAL SCIENCES （ 影响因子：5.9； ）

ISSN： 0570-1783

年卷期： 2023 年 68 卷 2 期

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收录情况： SCI

摘要： Deoxynivalenol (DON), a toxic secondary metabolite produced by Fusarium species that mainly infect cereals, causes huge economic loss to the agricultural industry. Biodegradation has emerged as a promising method owing to its high efficiency, specificity, and environment-friendly. The present study reports the procedures for obtaining a bacterial consortium YM-1 from the intestines of free-range chickens by anaerobic culture in Luria-Bertani (LB) and 96-well plates. The YM-1, mainly composed of Proteus_vulgaris, Tissierella_praeacuta, Fusobacterium_varium, uncultured_bacterium_g_Peptoniphilus, Bacteroides_uniformis, Bacteroides_vulgatus, Bacteroides_thetaiotaomicron, Clostridium_cochlearium, and Eubacterium_limosum, was able to effectively de-epoxidize 50 gg/mL DON by 99.2 % within 24 h under an anaerobic condition. The viability of the intestinal por-cine epithelial cell line-J2 (IPEC-J2) was significantly damaged under 0.5 gg/mL, 1 gg/mL, or 2 gg/mL DON expo-sure. The products of DON degraded by YM-1, mainly consisting of de-epoxy-deoxynivalenol (DOM-1), were validated to be non-toxic to the IPEC-J2 cells. These results indicate the potential use of bacterial consortium YM-1 as a bio-agent for DON decontamination in foods and feeds.& COPY; 2023 Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/licenses/by-nc-nd/4.0/).

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