Improvement of collagen self-assembly and thermal stability in the presence of trehalose

文献类型： 外文期刊

第一作者： Zhang, Juntao

作者： Zhang, Juntao;Huang, Yaozhi;Xie, Lvqin;Wei, Benmei;Xu, Chengzhi;Xu, Yuling;Zheng, Mingming;Wang, Haibo;Liu, Wei;Zheng, Mingming

作者机构：

期刊名称：NEW JOURNAL OF CHEMISTRY （ 影响因子：3.925； 五年影响因子：3.629 ）

ISSN： 1144-0546

年卷期： 2022 年 46 卷 19 期

页码：

收录情况： SCI

摘要： Collagen self-assembly into fibrous structures is a phenomenon that attracts wide attention in the biomedical and tissue engineering material fields. However, collagen's shortcoming of low thermal stability limits its application. Trehalose, a well-known compatible osmolyte, can stabilize proteins against various denaturing conditions. Herein, the effect of trehalose on the self-assembly and conformational stability of collagen was explored. The effect of trehalose on self-assembly was studied with turbidity, chloramine T assay, rheology, SEM and TEM characterizations. Circular dichroism (CD) was used to monitor the impact of trehalose on the triple helical conformation stability of collagen. The obtained experimental results reveal that trehalose could (1) accelerate the self-assembly kinetics and improve the self-assembly degree of collagen and the mechanical properties of the resulting hydrogels, but has no impact on the microstructure of the resulting collagen fibrils, and (2) increase the thermal stability of collagen. Both are desirable for the industrial application of collagen-based materials. Furthermore, the MTT assay and cell morphology characterization suggest that trehalose has no effect on the cytocompatibility of collagen materials.

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