Toothpick DNA extraction combined with handheld LAMP microfluidic platform for simple and rapid meat authentication

文献类型： 外文期刊

第一作者： Xiao, Bin

作者： Xiao, Bin;Wang, Nan;Zhang, Juan;Sun, Xiaoyun;Chen, Jiaci;Huang, Fengchun;Chen, Ailiang;Zhou, Tianping;Wang, Junbo;Li, Nan;Zhou, Tianping;Wang, Junbo;Wang, Junbo

作者机构：

关键词： Toothpick; Meat adulteration; Handheld microfluidic chip; LAMP; Analyzer; App; Point-of-care testing

期刊名称：FOOD CHEMISTRY （ 影响因子：8.5； 五年影响因子：8.2 ）

ISSN： 0308-8146

年卷期： 2024 年 460 卷

页码：

收录情况： SCI

摘要： Adulteration of meat is a global issue, necessitating rapid, inexpensive, and simple on-site testing methods. Therefore, the present study aimed to develop a one-minute toothpick-based DNA extraction method, a handheld microfluidic chip, and a smartphone-controlled portable analyzer for detecting multiple meat adulterations. A toothpick was inserted into the meat to promote DNA release and adsorption. Furthermore, a handheld micro- fluidic chip was designed for DNA elution on toothpicks and fluid distribution. Finally, a smartphone-actuated portable analyzer was developed to function as a heater, signal detector, and result reader. The portable device comprises a microcontroller, a fluorescence detection module, a step scanning unit, and a heating module. The proposed device is portable, and the app is user-friendly. This simple design, easy operation, and fast- response system could rapidly detect as little as 1% of simulated adulterated samples (following UK standards) within 40 min at a cost of less than USD 1 per test.

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