Development of a TaqMan One-Step Quantitative PCR Assay for the Simultaneous Detection of Novel Goose Parvovirus and Novel Duck Reovirus

文献类型： 外文期刊

第一作者： Wang, Yimin

作者： Wang, Yimin;Bi, Zhuangli;Meng, Chunchun;Zhu, Jie;Liu, Guangqing;Li, Chuanfeng;Wang, Yimin;Wang, Yong;Wang, Jinbin;Wang, Gang;Ru, Xin

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关键词： novel goose parvovirus; novel duck reovirus; simultaneous detection; one-step qPCR

期刊名称：MICROORGANISMS （ 影响因子：4.2； 五年影响因子：4.6 ）

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年卷期： 2025 年 13 卷 7 期

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收录情况： SCI

摘要： The novel goose parvovirus (NGPV) and the novel duck reovirus (NDRV) are pathogens that can substantially affect the growth and development of ducklings, causing considerable economic losses to duck farms. Therefore, a timely, rapid, accurate, and high-throughput diagnosis and identification of viral infections are critical for preventing the spread of epidemics. In this study, a TaqMan probe-based duplex one-step RT-qPCR was established for the simultaneous detection and qualitative and quantitative identification of the two viruses. It demonstrated greater sensitivity than conventional PCR, detecting as low as 2.42 copies/mu L of NGPV genome and 70.1 copies/mu L of NDRV genome. Additionally, it exhibited remarkable specificity, responding exclusively to the nucleic acids of target pathogens. It also demonstrated excellent reproducibility and availability, particularly in clinical settings, with a coinfection detection rate of 13.3%, contributing to the development of NGPV- and NDRV-testing technologies.

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