Activation of Gut Microbiota-HIF1α Axis Effectively Restores Resistance to Aeromonas veronii Caused by Improper Administration of AiiO-AIO6

文献类型： 外文期刊

第一作者： Yang, Hongwei

作者： Yang, Hongwei;Xia, Rui;Meng, Delong;Li, Shenghui;Wang, Tiantian;Zhou, Zhigang;Yang, Hongwei;Li, Shengkang;Xia, Rui;Teame, Tsegay;Ding, Qianwen;Yao, Yuanyuan;Xu, Xiaoqing;Yang, Yalin;Ran, Chao;Zhang, Zhen;Teame, Tsegay;Zhang, Yaqing;Niemann, Benjamin;Guan, Le Luo;Zhang, Zhen

作者机构：

关键词： quenching enzyme; prebiotic; Bacillus subtilis; hif1 alpha; gut microbiota

期刊名称：JOURNAL OF NUTRITION （ 影响因子：3.8； 五年影响因子：4.2 ）

ISSN： 0022-3166

年卷期： 2025 年 155 卷 5 期

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收录情况： SCI

摘要： Background: Feeding adult zebrafish a diet supplemented with quenching enzyme AiiO-AIO6 (AIO6) for 3 wk improved the growth performance and disease resistance. However, when the feeding period was extended to 8 wk, zebrafish's disease resistance to Aeromonas veronii decreased. Objectives: We investigated the mechanisms of the reduced disease resistance of zebrafish induced by feeding on an AIO6 supplemented diet for a long term (8 wk) and assessed the effectiveness of feed additives in restoring the low disease resistance. Methods: One-month-old (adult) zebrafish were fed with a basal diet and the basal diet supplemented with AIO6 (10 U/g) for 8 wk (experiment 1). Furthermore, the zebrafish larvae model (experiment 2) was developed and used to study the mechanisms of how AIO6 affected disease resistance (experiment 3). We also investigated the effectiveness of selected prebiotic tributyrin, beta-glucan or mannan in activating gut microbiota-HIF1 alpha to restore the low disease resistance of adult zebrafish fed with AIO6 for 8 wk (experiment 4). Lastly, the effects of Bacillus subtilis in activating the gut microbiota-HIF1 alpha and improving the low disease resistance of zebrafish larvae induced by AIO6 were examined (experiment 5). Results: Feeding adult zebrafish with AIO6 for 8 wk promoted growth but disordered the gut microbiota and reduced disease resistance. The zebrafish larvae model confirmed that feeding AIO6 for 2 d increased disease resistance, whereas 7 d decreased the resistance by suppressing HIF1 alpha. Using a germ-free zebrafish larvae model, we also demonstrated that AIO6-induced gut microbiota mediated inhibition of HIF1 alpha. Furthermore, zebrafish fed on the AIO6-containing diet supplement with tributyrin, beta-glucan, mannan, or Bacillus subtilis activated the gut microbiota-HIF1 alpha axis to reverse the low resistance caused by AIO6. Conclusions: Activating the gut microbiota-HIF1 alpha axis has a vital role in improving intestinal health and restores the low resistance to Aeromonas veronii caused by improper administration of dietary AIO6 in zebrafish.

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