Exposure of zebrafish (Danio rerio) to trans-2-hexenal induces oxidativestress and protein degeneration of the gill br

文献类型： 外文期刊

第一作者： Ma, Dicheng

作者： Ma, Dicheng;Yu, Haiyan;Cui, Guangrui;Zhu, Jiamei;Mu, Wei;Liu, Feng;Zhu, Bingyu

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关键词： Trans-2-hexenal; Adult zebrafish; Gill; Oxidative stress; Protein degeneration

期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：10.753； 五年影响因子：10.237 ）

ISSN： 0048-9697

年卷期： 2023 年 854 卷

页码：

收录情况： SCI

摘要： Trans-2-hexenal (T2H) has great commercial value for development as a biopesticide, but its toxicity risk to nontargetorganisms is unknown. Here, the toxicity and underlying mechanism of T2H on zebrafish (Danio rerio) were investigated.The LC50(48 h) of T2H on zebrafish is 4.316 mu g/mL, and the aldehyde group is essential to its toxicity. In 14-day chronictoxicity tests, 0.432 mu g/mL T2H resulted in a higher mortality of zebrafish than the control group. Furthermore, the sen-sitivity of zebrafish to different administration methods was gill administration>oral administration>transdermaladministration>intravenous injection. T2H induced significant cell death and ROS generation in zebrafish gill cells in aconcentration-dependent manner. After treatment with 4.316 mu g/mL T2H, the expression of oxidative stress-relatedgenes (nrf2,gstp1,keap1b,sod1andsod2) and the content of malondialdehyde (MDA) were up-regulated. Incubationwith T2H caused an immediate denaturation of gill protein, which was aggravated with increasing dose of T2H. Wealso found that T2H at 21.225 mg/mL significantly reduced thein vitroactivity of succinate dehydrogenase (SDH).Among the three amino acids tested, T2H was only found to react with methionine and glycine to form adducts, whichmay be the basis of the protein denaturation. This study confirmed that T2H could induce oxidative stress and protein de-naturation in zebrafish gills, providing important information for risk assessment of T2H exposure.

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