Insight rifampicin-resistant (rpoB) mutation in Pseudomonas stutzeri leads to enhance the biosynthesis of secondary metabolites to survive against harsh environments

文献类型： 外文期刊

第一作者： Alam, Khandakar Mohiul

作者： Alam, Khandakar Mohiul;Yan, Yongliang;Lin, Min;Islam, Md. Ariful;Gaber, Ahmed;Hossain, Akbar

作者机构：

关键词： Nitrogenase activity; Rifampicin; RNA polymerase beta subunit; RpoB gene; Pseudomonas stutzeri

期刊名称：ARCHIVES OF MICROBIOLOGY （ 影响因子：2.667； 五年影响因子：2.729 ）

ISSN： 0302-8933

年卷期： 2022 年 204 卷 7 期

页码：

收录情况： SCI

摘要： In this study, a wild-type and five distinct rifampicin-resistant (Rif(r)) rpoB mutants of Pseudomonas stutzeri (i.e., Q518R, D521Y, D521V, H531R and 1614T) ability were investigated against harsh environments (particularly nutritional complexity). Among these, the robust Rif(r) phenotype of P. Stutzeri was associated only with base replacements of the amino deposits. The use of carboxylic and amino acids significantly increased in various Rif(r) mutants than that of wild type of P. stutzeri. The assimilation of carbon and nitrogen (N) sources of Rif(r) mutants' confirmed that the organism maintains the adaptation in nutritionally complex environments. Acetylene reduction assay at different times also found the variability for N-fixation in all strains. Among them, the highest nitrogenase activity was determined in mutant 'D521V'. The assimilation of carbon and nitrogen sources of P. stutzeri and its Rif(r) mutants ensures that the organism maintains the adaptability in nutritionally complex environments through fixing more nitrogen.

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