Identification and Functional Evaluation of Three Polyubiquitin Promoters from Hevea brasiliensis
文献类型： 外文期刊
第一作者： Xin, Shichao
作者： Xin, Shichao;Udayabhanu, Jinu;Dai, Xuemei;Hua, Yuwei;Fan, Yueting;Huang, Huasun;Huang, Tiandai;Xin, Shichao;Udayabhanu, Jinu;Dai, Xuemei;Hua, Yuwei;Fan, Yueting;Huang, Huasun;Huang, Tiandai
作者机构：
关键词： rubber tree; constitutive promoter; cis-acting elements; Agrobacterium-mediated transformation; protoplast transformation; transgene expression
期刊名称：FORESTS （ 影响因子：3.282； 五年影响因子：3.292 ）
ISSN：
年卷期： 2022 年 13 卷 6 期
页码：
收录情况： SCI
摘要： Hevea brasiliensis is an economically important tree species that provides the only commercial source of natural rubber. The replacement of the CaMV35S promoter by endogenous polyubiquitin promoters may be a viable way to improve the genetic transformation of this species. However, no endogenous polyubiquitin promoters in Hevea have been reported yet. Here, we identified three Hevea polyubiquitin genes HbUBI10.1, HbUBI10.2 and HbUBI10.3, which encode ubiquitin monomers having nearly identical amino acid sequences to that of AtUBQ10. The genomic fragments upstream of these HbUBI genes, including the signature leading introns, were amplified as putative HbUBI promoters. In silico analysis showed that a number of cis-acting elements which are conserved within strong constitutive polyubiquitin promoters were presented in these HbUBI promoters. Transcriptomic data revealed that HbUBI10.1 and HbUBI10.2 had a constitutive expression in Hevea plants. Semi-quantitative RT-PCR showed that these three HbUBI genes were expressed higher than the GUS gene driven by CaMV35S in transgenic Hevea leaves. All three HbUBI promoters exhibited the capability to direct GFP expression in both transient and stable transformation assays, although they produced lower protoplast transformation efficiencies than the CaMV35S promoter. These HbUBI promoters will expand the availability of promoters for driving the transgene expression in Hevea genetic engineering.
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