Biological studies reveal the role of trpA gene in biofilm formation, motility, hemolysis and virulence in Vibrio anguillarum

文献类型： 外文期刊

第一作者： Che, Jinyuan

作者： Che, Jinyuan;Wang, Lei;Liu, Binghong;Fang, Qitong;Nissa, Meher un;Bao, Baolong;Luo, Tuyan;Bao, Baolong

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关键词： Vibrio anguillarum; trpA; Biofilm formation; Motility; Hemolysin; Virulence

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.5； 五年影响因子：3.6 ）

ISSN： 0882-4010

年卷期： 2025 年 200 卷

页码：

收录情况： SCI

摘要： Vibrio anguillarum is a pathogen responsible for vibriosis in aquaculture animals. The formation of bacterial biofilm contributes to infections and increases resistance to antibiotics. Tryptophanase and its substrate tryptophan have been recognized as signal molecules regulating bacterial biofilm formation. This study aimed to investigate the role of the trpA gene encoding tryptophan synthase in V. anguillarum through constructing a trpA mutant (ztrpA) and its complemented strain (CztrpA). The ztrpA produced less tryptophan compared to the WT and ztrpA strains, with almost no detectable indole synthesis in the ztrpA mutant. RNAseq analysis showed that 152 genes were differentially expressed in the ztrpA mutant, including 64 upregulated and 88 downregulated genes. KEGG enrichment analysis and qRT-PCR validation indicated that genes associated with bacterial chemotaxis, two component system, quorum sensing and biofilm formation were downregulated. Crystal violet staining confirmed that the ztrpA mutant decreased biofilm formation due to the reduced tryptophan and indole. Our studies also showed that TrpA plays an important role in the motility and hemolytic ability of V. anguillarum. Finally, the virulence of the ztrpA mutant was evaluated using a Tetrahymena infection model, which demonstrated that the virulence of ztrpA mutant was significantly attenuated. These findings provide insight into the role of trpA gene in biofilm formation, motility, hemolysis, and virulence in V. anguillarum.

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