Phenotypic characteristics and protective efficacy of an attenuated Mycoplasma hyopneumoniae vaccine by aerosol administration

文献类型： 外文期刊

第一作者： Hao, Fei

作者： Hao, Fei;Bai, Yun;Xie, Xing;Yuan, Ting;Wei, Yanna;Xiong, Qiyan;Gan, Yuan;Zhang, Lei;Zhang, Zhenzhen;Shao, Guoqing;Feng, Zhixin;Hao, Fei;Bai, Yun;Xie, Xing;Shao, Guoqing;Feng, Zhixin

作者机构：

关键词： Mycoplasma hyopneumoniae; Aerosol vaccination; Ultrasonic atomizer; Phenotypic characteristics; Protective efficacy

期刊名称：VACCINE （ 影响因子：4.169； 五年影响因子：4.27 ）

ISSN： 0264-410X

年卷期： 2022 年 40 卷 42 期

页码：

收录情况： SCI

摘要： With the improvement of large-scale breeding in pig farms, conventional head-by-head immunization has disadvantages with low efficiency and high cost. Considering that most pathogens leading to pul-monary diseases circulate from the respiratory mucosa, immunization through the respiratory tract route has been a highly attractive vaccine delivery strategy. In this study, to develop an effective Mycoplasma hyopneumoniae (Mhp) aerosol vaccine, a customized ultrasonic atomizer was developed. The aerody-namic diameter, activity, and content of the Mhp aerosol vaccine were measured. In addition, piglets were immunized with the Mhp aerosol vaccine, and the immunity of the animal challenge protection test was evaluated. At the end of nebulization, the mass median aerodynamic diameters (MMAD) and geo-metric standard deviation (GSD) of the aerosol were 2.98 +/- 0.02 lm and 1.51 +/- 0.02, respectively. Moreover, 10 min after nebulization, the MMAD and GSD of the aerosol were 2.76 +/- 0.02 lm and 1.51 +/- 0.01, respectively, which were hardly changed. Compared with theoretical value, the actual titer of aerosol vaccines presented in 50% color changing unit (CCU50) after nebulization decreased 0.6. The shape, size, and uniformity of collected aerosols are relatively stable. The proportion of Mhp in aerosol produced by vaccine stock solution and 10 times diluted vaccine solution was 76.52% and 58.82%, respec-tively, and the average number of Mhp in a single aerosol was 3.06 and 1.51, respectively. In addition, the aerosol vaccine antigen particles could be transported to the lower respiratory tract, a local mucosal immune response was induced in piglets. The vaccine colonized the respiratory tract and significantly decline the lung lesion index after aerosol vaccination. In conclusion, an effective aerosol vaccine against Mhp infection was developed. And this is the first effective assessment for Mhp live vaccine with aerosolization against infection in piglets.(c) 2022 Elsevier Ltd. All rights reserved.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序