Characterization of an α-Amylase from the Honeybee Chalk Brood Pathogen Ascosphaera apis

文献类型： 外文期刊

第一作者： Li, Jincheng

作者： Li, Jincheng;Liu, Sen;Yang, Chenjie;Pu, Huili;Lin, Longbin;Li, Xiaoxia;Zhang, Liaoyuan;Chen, Yuxi;Qiu, Junzhi;Keyhani, Nemat O.;Jia, Peisong;Wu, Dongmei;Pan, Jieming;Stevenson, Philip C.;Fernandez-Grandon, G. Mandela;Guan, Xiayu

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关键词： fungal pathogen; Ascosphaera apis; alpha-amylase; medium factor optimization; purification; large-scale production

期刊名称：JOURNAL OF FUNGI （ 影响因子：4.7； 五年影响因子：5.2 ）

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年卷期： 2023 年 9 卷 11 期

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收录情况： SCI

摘要： The insect pathogenic fungus, Ascosphaera apis, is the causative agent of honeybee chalk brood disease. Amylases are secreted by many plant pathogenic fungi to access host nutrients through the metabolism of starch, and the identification of new amylases can have important biotechnological applications. Production of amylase by A. apis in submerged culture was optimized using the response surface method (RSM). Media composition was modeled using Box-Behnken design (BBD) at three levels of three variables, and the model was experimentally validated to predict amylase activity (R-2 = 0.9528). Amylase activity was highest (45.28 +/- 1.16 U/mL, mean +/- SE) in media composed of 46 g/L maltose and1.51 g/L CaCl2 at a pH of 6.6, where total activity was similar to 11-fold greater as compared to standard basal media. The enzyme was purified to homogeneity with a 2.5% yield and 14-fold purification. The purified enzyme had a molecular weight of 75 kDa and was thermostable and active in a broad pH range (> 80% activity at a pH range of 7-10), with optimal activity at 55 degrees C and pH = 7.5. Kinetic analyses revealed a K-m of 6.22 mmol/L and a V-max of 4.21 mu mol/mL.min using soluble starch as the substrate. Activity was significantly stimulated by Fe2+ and completely inhibited by Cu2+, Mn2+, and Ba2+ (10 mM). Ethanol and chloroform (10% v/v) also caused significant levels of inhibition. The purified amylase essentially exhibited activity only on hydrolyzed soluble starch, producing mainly glucose and maltose, indicating that it is an endo-amylase (alpha-amylase). Amylase activity peaked at 99.38 U/mL fermented in a 3.7 L-bioreactor (2.15-fold greater than what was observed in flask cultures). These data provide a strategy for optimizing the production of enzymes from fungi and provide insight into the alpha-amylase of A. apis.

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