Single-Cell Atlas of Spleen Remodeling Reveals Macrophage Subset-Driven ASFV Pathogenesis

文献类型： 外文期刊

第一作者： Wang, Liyuan

作者： Wang, Liyuan;Sun, Shouzhang;Liu, Lei;Chen, Yun;Tang, Zhonglin;Wang, Liyuan;Sun, Shouzhang;Liu, Lei;Chen, Yun;Tang, Zhonglin;Sun, Shouzhang;Liu, Lei;Zheng, Haixue;Zheng, Haixue;Zheng, Haixue;Tang, Zhonglin

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关键词： ASFV; scRNA-seq; macrophage subsets; viral pathogenesis

期刊名称：BIOLOGY-BASEL （ 影响因子：3.5； 五年影响因子：4.0 ）

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年卷期： 2025 年 14 卷 7 期

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收录情况： SCI

摘要： African swine fever virus (ASFV) causes global swine outbreaks, but its cellular pathogenesis is poorly understood. Using single-cell RNA data from ASFV-infected pig spleens across four timepoints, we identified macrophages as the primary viral reservoir, with infection driving lymphoid depletion and myeloid expansion. We characterized four functionally distinct macrophage subsets, including a metabolically reprogrammed SusceptibleMac population serving as the major viral niche and an AntiviralMac subset rapidly depleted during infection. Viral gene expression analysis revealed E165R as a central hub in viral replication networks, while host transcriptomics uncovered disruption of Netrin signaling pathways that may facilitate immune evasion. Pseudotime analysis revealed dynamic macrophage state transitions during infection. These findings provide a high-resolution cellular atlas of ASFV pathogenesis, revealing macrophage subset-specific responses that shape disease outcomes and identifying potential targets for therapeutic intervention.

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