Double-Stranded Break Repair in Mammalian Cells and Precise Genome Editing

文献类型： 外文期刊

第一作者： Ali, Akhtar

作者： Ali, Akhtar;Xiao, Wei;Bi, Yanzhen;Ali, Akhtar;Babar, Masroor Ellahi

作者机构：

关键词： DSB repair; NHEJ; HR; RNA template; genome editing

期刊名称：GENES （ 影响因子：4.141； 五年影响因子：4.474 ）

ISSN：

年卷期： 2022 年 13 卷 5 期

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收录情况： SCI

摘要： In mammalian cells, double-strand breaks (DSBs) are repaired predominantly by error-prone non-homologous end joining (NHEJ), but less prevalently by error-free template-dependent homologous recombination (HR). DSB repair pathway selection is the bedrock for genome editing. NHEJ results in random mutations when repairing DSB, while HR induces high-fidelity sequence-specific variations, but with an undesirable low efficiency. In this review, we first discuss the latest insights into the action mode of NHEJ and HR in a panoramic view. We then propose the future direction of genome editing by virtue of these advancements. We suggest that by switching NHEJ to HR, full fidelity genome editing and robust gene knock-in could be enabled. We also envision that RNA molecules could be repurposed by RNA-templated DSB repair to mediate precise genetic editing.

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